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Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums

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Abstract

Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine, and it is related to the excessive generation of reactive oxygen species (ROS). Photobiomodulation (PBM) can promote wound healing in many ways, so it can be used as a method for the treatment of delayed healing of DM wounds. In this study, we investigated the effect of PBM on ROS homeostasis in human embryonic skin fibroblast cells (CCC-ESFs) cultured in high glucose concentrations. The CCC-ESFs were cultured in vitro and divided into two groups, including the control group and the 635 nm laser irradiation group. After 2 days of high glucose treatment, the experimental group was irradiated with different doses of laser for 3 days. First, we measured the cellular proliferation, and the results showed that laser irradiation could promote cellular proliferation. Then, we measured the generation of ROS, the activities of total superoxide dismutase (SOD), and total antioxidant capacity (TAC) of the cells; the results showed that high glucose destroyed cells by inducing high concentration of ROS, the balance of oxidation, and antioxidation cause oxidative stress damage to cells. PBM can increase the antioxidant capacity of cells, reducing the high concentration of ROS induced by high glucose. Finally, we measured the levels of mitochondrial membrane potential (∆ψm) and the secretion of nuclear factor kappa-B (NF-κB), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β); the results showed that PBM can reduce apoptosis and regulate the inflammatory state. We conclude that PBM can maintain the ROS homeostasis, increase the TAC of cells, and trigger the cellular proliferation, and the response of CCC-ESFs to PBM was dose-dependent.

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Funding

This work was funded by the National Natural Science Foundation of China (Grant Nos. 61705164, 61905177) and the Tianjin Natural Science Foundation of China (Grant Nos. 19JCQNJC01600, 19JCQNJC01400).

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Authors and Affiliations

  1. School of Life Sciences, Tiangong University, Xiqing District, Binshui West Road, Tianjin, 300387, China

    Hongli Chen, Mengru Tu, Jia Shi, Yunhao Wang, Zhenhao Hou & Jinhai Wang

  2. Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin, 300072, China

    Hongli Chen

  3. Tianjin Photoelectric Detection Technology and Systems Key Laboratory, Tianjin, 300387, China

    Hongli Chen, Jia Shi & Jinhai Wang

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  1. Hongli Chen
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Correspondence to Jinhai Wang.

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Chen, H., Tu, M., Shi, J. et al. Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums. Lasers Med Sci 36, 555–562 (2021). https://doi.org/10.1007/s10103-020-03057-4

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  • DOI: https://doi.org/10.1007/s10103-020-03057-4

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