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Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar)

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Abstract

The reconstructive techniques have been widely used in Veterinary Medicine. The post-operative adjuvants therapies like the low-level laser therapy (LLLT) are used to decrease inherent complications to reconstructive surgeries. This article purposed to define the LLLT effects on the healing, inflammation, and vascularization of the skin grafts in applicable time intervals to veterinary surgical routine. Forty rats (Rattus norvegicus albinus wistar) were used and each one was submitted to autogenous cutaneous mesh grafting in the interescapular region. The rats were randomly distributed in five groups (G1, G2, G3, G4, and G5) in accordance with the 6 J/cm2 or 10 J/cm2 dose every 3 or 5 days. These treatments were applied on the skin graft for 15 days. The histochemical evaluation with Picrosirius showed greater expression of collagen type 1 – red in grafts of G5 (p < 0.05), while in G1 did not; the expression of collagen type III – green was not induced by LLLT. The histochemical evaluation with hematoxylin-eosin showed greater numbers of fibroblasts in grafts of G4 (p < 0.05) and less hemorrhage in grafts of G5 (p < 0.05). There was modulation of the inflammatory response in irradiated skin grafts. It is concluded the exhibition of the skin grafts to 6 J/cm2 or 10 J/cm2 dose every 5 days improved the healing and the modulation of the local inflammation.

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Funding

This study was funded by São Paulo Research Foundation (FAPESP) research fellowship to Moreira SH (Protocol 2017/25951-9).

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Authors and Affiliations

  1. Departamento de Clínica e Cirurgia Veterinária, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brasil

    Stella H. Moreira, Jorge L. G. Álvarez, Pedro C. Cassino, Caio C. Bustamante, Filippo J. L. Bernardes, Caroline Y. Kajiura & Andrigo B. De Nardi

  2. Departamento de Clínica e Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de acesso Professor Paulo Donato Castellane, s/n, Jaboticabal, São Paulo, CEP 14884-900, Brazil

    Stella H. Moreira

  3. Centro de Pesquisa em Oncologia Molecular, Hospital do Câncer de Barretos, Barretos, São Paulo, Brasil

    Josiane M. Pazzini

Authors
  1. Stella H. Moreira
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  2. Josiane M. Pazzini
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  3. Jorge L. G. Álvarez
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  5. Caio C. Bustamante
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  6. Filippo J. L. Bernardes
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  7. Caroline Y. Kajiura
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  8. Andrigo B. De Nardi
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Corresponding author

Correspondence to Stella H. Moreira.

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The study followed the guidelines of the Animal Experimentation Code of Ethics of the Brazilian College of Animal Experimentation and was approved by the Ethics Committee of Animals Use (CEUA) from FCAV, Unesp (Protocol 010401/17).

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The authors declare that they have no conflict of interest.

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Moreira, S.H., Pazzini, J.M., Álvarez, J.L.G. et al. Evaluation of angiogenesis, inflammation, and healing on irradiated skin graft with low-level laser therapy in rats (Rattus norvegicus albinus wistar). Lasers Med Sci 35, 1103–1109 (2020). https://doi.org/10.1007/s10103-019-02917-y

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  • DOI: https://doi.org/10.1007/s10103-019-02917-y

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