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Dual effect of blue light on Fusariumsolani clinical corneal isolates in vitro

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Abstract

The purpose was to investigate the effect of daylight-intensity blue light on F. solani isolated from the cornea of patients with fungal keratitis. Spore suspensions of 5 F. solani strains (one standard strain and 4 clinical corneal isolates) were prepared in 6-well plates. Blue light groups were irradiated by a light-emitting diode (LED) device with a peak wavelength of 454 nm at 0.5 mW/cm2 for 0 to 48 h, while the controls were maintained in darkness. Hyphal morphology in the 6-well plates was recorded at 0, 12, 24, 36, 48 h. One hundred microliters of spore suspensions of each strain at these five time points was transferred to SGA plates and cultured for 36 h at 29 °C; the number of colonies formed was counted as a measure of conidia quality and viability. Blue light has dual effects on F. solani. The hyphal length of F. solani exposed to blue light was significantly shorter than that of the control (P < 0.01), indicating that fungal growth was inhibited. Meanwhile, instead of reducing the viability of spores, blue light significantly enhanced the conidia quality and viability after at least 24 h irradiation. Daylight-intensity blue light exposure will inhibit the hyphal growth of F. solani but promote conidiation, which would be more harmful to fungal keratitis. Eliminating the influence of blue light for these patients should be taken into account.

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Funding

This work is supported by National Natural Science Foundation of China (81670821).

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Authors and Affiliations

  1. Department of Ophthalmology, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China

    Yingyu Li, Pei Zhang, Chen Huang & Wei Wang

  2. Medical Research Center, Peking University Third Hospital, Beijing, China

    Chen Huang

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  1. Yingyu Li
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  2. Pei Zhang
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  3. Chen Huang
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  4. Wei Wang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Li Yingyu and Zhang Pei. The first draft of the manuscript was written by Li Yingyu and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wei Wang.

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Li, Y., Zhang, P., Huang, C. et al. Dual effect of blue light on Fusariumsolani clinical corneal isolates in vitro. Lasers Med Sci 35, 1299–1305 (2020). https://doi.org/10.1007/s10103-019-02911-4

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