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Metabolic activity of odontoblast-like cells irradiated with blue LED (455 nm)

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Abstract

Blue light emitting diodes (LEDs) are frequently used in dentistry for light activation of resin-based materials; however, their photobiostimulatory effects have not yet been fully investigated. This study aimed to investigate the effect of blue LED (455 nm) on the metabolism of odontoblast-like cells MDPC-23. Energy doses of 2 and 4 J/cm2 were used at 20 mW/cm2 fixed power density. MDPC-23 cells were seeded at 10,000 cells/cm2 density in Dulbecco’s modified Eagle’s medium (DMEM) containing 10 % fetal bovine serum (FBS). After 12 h, the culture medium was replaced with new DMEM supplemented with 0.5 % of FBS, and the cells were incubated for further 12 h. After that, single irradiation was performed to the culture, under selected parameters. Cell viability evaluations (Alamar Blue Assay, n = 12), number of viable cells (Trypan Blue Assay, n = 12), morphological analysis by scanning electron microscopy (SEM, n = 2), gene expression (n = 6) of alkaline phosphatase (Alp), collagen (Col-1a1), and dental matrix protein (Dmp-1) (quantitative polymerase chain reaction (qPCR)) were performed 72 h after irradiation. Data were analyzed by Kruskal-Wallis, ANOVA, and Tukey tests (p < 0.05). Direct light application at 4 J/cm2 energy dose had no negative effects on cell viability, while irradiation with 2 J/cm2 reduced cell metabolism. None of doses affected the number of viable cells compared with the control group. The two energy doses downregulated the expression of Alp; however, expression of Col-1a1 and Dmp-1 had no alteration. Cells presented change in the cytoskeleton only when irradiated with 2 J/cm2. In conclusion, the blue LED (455 nm) irradiation, under the evaluated parameters, had no biostimulatory effects on MDPC-23 cells.

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Acknowledgements

The authors acknowledge with thanks the financial support provided by the Brazilian financing agency FAPESP (São Paulo State Research Supporting Foundation-grant 2012/17552-2).

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Authors and Affiliations

  1. Department of Restorative Dentistry, Araraquara Dental School, “Univ. Estadual Paulista-UNESP”, Araraquara, SP, Brazil

    Leopoldina Fátima Dantas de Almeida

  2. Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, “Univ. Estadual Paulista-UNESP”, Rua Humaitá, 1680, Araraquara, SP, Brazil

    Fernanda Gonçalves Basso, Ana Paula Silveira Turrioni & Josimeri Hebling

  3. Department of Physiology and Pathology, Araraquara Dental School, “Univ. Estadual Paulista-UNESP”, Araraquara, SP, Brazil

    Carlos Alberto de-Souza-Costa

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  1. Leopoldina Fátima Dantas de Almeida
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  2. Fernanda Gonçalves Basso
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  3. Ana Paula Silveira Turrioni
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Correspondence to Josimeri Hebling.

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de Almeida, L.F.D., Basso, F.G., Turrioni, A.P.S. et al. Metabolic activity of odontoblast-like cells irradiated with blue LED (455 nm). Lasers Med Sci 31, 119–125 (2016). https://doi.org/10.1007/s10103-015-1837-z

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  • DOI: https://doi.org/10.1007/s10103-015-1837-z

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