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Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat’s TMJ induced by carrageenan

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Abstract

Temporomandibular disorders (TMDs) are mostly inflammatory conditions widespread in the population. Previous studies have shown positive effects of either laser or light-emitting diode (LED) phototherapies on treating TMDs, but their action and mechanism in the inflammatory infiltrate of the temporomandibular joint are still poorly understood. The aim of this study was to assess, through histological analysis, the effectiveness of using laser light (λ 780 nm, 70 mW, continous wave (CW), 10 J) and LED (λ 850 ± 10 nm, 100 mW, CW, 10 J) on the inflammation of the temporomandibular joint of rats induced by carrageenan. Forty-five animals were divided into three groups with five animals each according to the experimental times of 2, 3, and 7 days: inflammation, inflammation+laser phototherapy, and inflammation+LED phototherapy. The first irradiation was performed 24 h after induction with an interval of 48 h between sessions. After animal death, specimens were processed and stained with hematoxylin-eosin (HE) and picrosirius. Then, the samples were examined histologically. Data were statistically analyzed. The inflammation group showed mild to moderate chronic inflammatory infiltrate between bone trabecules of the condyle. Over the time course of the study in the laser group, the region of the condyle presented mild chronic inflammation and intense vascularization. In the LED group, the condyle showed aspects of normality and absence of inflammation in some specimens. In all the time points, the laser-irradiated groups showed greater amount of collagen deposition in the condyle (p = 0.04) and in the disc (p = 0.03) when compared to the inflammation and LED groups, respectively. Laser- and LED-treated groups demonstrate a smaller number of layers of the synovial membrane when compared to the non-irradiated groups. It was concluded that, in general, laser and LED phototherapies resulted in a reduction of inflammatory infiltrate in the temporomandibular joint of rat.

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Acknowledgments

We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for providing financial support for this project.

Conflicts of interest

The authors received a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), a government research agency, but have full control of all primary data and agree to allow the journal to review their data if requested.

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Authors and Affiliations

  1. Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Isabele C. V. de Castro, Cristiane B. Rosa, Carolina M. Carvalho, Juliana S. Aragão & Antonio L. B. Pinheiro

  2. Dental Public Health, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, CEP 40110-150, Brazil

    Maria Cristina T. Cangussu

  3. Oral Pathology School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil

    Jean N. dos Santos

  4. University of Camilo Castelo Branco Núcleo do Parque Tecnológico de São José dos Campos, Rod. Presidente Dutra Km 139, Eugênio de Melo, São José dos Campos, SP, 12247-004, Brazil

    Antonio L. B. Pinheiro

  5. National Institute of Optics and Photonics, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, 13560-970, Brazil

    Antonio L. B. Pinheiro

Authors
  1. Isabele C. V. de Castro
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  2. Cristiane B. Rosa
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  7. Antonio L. B. Pinheiro
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Correspondence to Antonio L. B. Pinheiro.

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de Castro, I.C.V., Rosa, C.B., Carvalho, C.M. et al. Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat’s TMJ induced by carrageenan. Lasers Med Sci 30, 2105–2113 (2015). https://doi.org/10.1007/s10103-015-1748-z

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  • DOI: https://doi.org/10.1007/s10103-015-1748-z

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