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Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis

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Abstract

Erbium-doped yttrium aluminum garnet (Er:YAG) laser treatment has demonstrated favorable wound healing effect after periodontal therapy. One of the reasons may be the positive biological effect of the low-level laser on the irradiated tissues, although the mechanism remains unclear. The aim of this study was to investigate the effect of low-level Er:YAG laser irradiation on cell proliferation and laser-induced differential expression of proteins in human gingival fibroblasts (HGFs) by proteomic analysis. In the first experiment, HGFs were exposed to low-level Er:YAG laser irradiation and the laser-induced cell proliferation and damage were evaluated on day 3. In the second experiment, proteomic analysis was performed on day 1 after irradiation. The peptides prepared from HGFs were analyzed by a hybrid ion trap-Fourier transform mass spectrometer, Mascot search engine, and UniProtKB database. A significant increase in cell proliferation without cell damage after irradiation was observed. Among the total identified 377 proteins, 59 proteins, including galectin-7, which was associated with the process of wound healing, were upregulated and 15 proteins were downregulated in laser-treated HGFs. In the third experiment, the increase in messenger RNA (mRNA) and protein expression of galectin-7 in the irradiated HGFs was validated by various analytical techniques. In addition, the effect of recombinant human galectin-7 on the modulation of HGFs proliferation was confirmed. The results indicate that low-level Er:YAG laser irradiation can promote HGF proliferation and induce a significant change in protein expression and the upregulation of galectin-7 expression may partly contribute to the increase in cell proliferation.

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Acknowledgments

This study was partially supported by the grant of the Global Center of Excellence Program of the International Research Center for Molecular Science in Tooth and Bone Diseases at TMDU funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan and the grant of Grants-in-Aid for Scientific Research (C) (no. 22392308 and no. 23463212 for A.A.) from the Japan Society for the Promotion of Science. We would like to acknowledge Dr. Kengo Iwasaki and Dr. Yoichi Taniguchi at TMDU for their kind advices and supports.

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Authors and Affiliations

  1. Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Mayumi Ogita, Akira Aoki, Masanori Sawabe, Hiromi Nanbara, Hiroaki Kobayashi, Yasuo Takeuchi, Koji Mizutani & Yuichi Izumi

  2. Department of Molecular Diagnosis (F8), Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Sachio Tsuchida, Mamoru Satoh & Fumio Nomura

  3. Chemical Analysis Center, Chiba University, Chiba, Japan

    Sayaka Kado

  4. Maxillofacial Orthognathics, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, TMDU, Bunkyo-ku, Tokyo, Japan

    Yoshiyuki Sasaki

  5. Global Center of Excellence Program of the International Research Center for Molecular Science in Tooth and Bone Diseases, Graduate School of Medical and Dental Sciences, TMDU, Bunkyo-ku, Tokyo, Japan

    Mayumi Ogita & Yuichi Izumi

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  1. Mayumi Ogita
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  2. Sachio Tsuchida
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  3. Akira Aoki
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Correspondence to Sachio Tsuchida or Akira Aoki.

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Table 1

LC-MS/MS-identified 377 proteins in HGFs with and/or without low-level Er:YAG laser irradiation (PDF 185 kb)

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Ogita, M., Tsuchida, S., Aoki, A. et al. Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis. Lasers Med Sci 30, 1855–1866 (2015). https://doi.org/10.1007/s10103-014-1691-4

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