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Effects of laser irradiation at different wavelengths (660, 810, 980, and 1,064 nm) on mucositis in an animal model of wound healing

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Abstract

The aim of the present study was to compare the effectiveness of four different laser wavelengths (660, 810, 980, and 1,064 nm) used for low-level laser therapy (LLLT) on the healing of mucositis in an animal model of wound healing by investigating the expression of platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-β), and blood-derived fibroblast growth factor (bFGF). Thirty-five male Wistar albino rats with a weight of 250–300 g body mass and 5 months old were used in the study. All animals were intraperitoneally injected with 100 mg/kg of 5-fluorouracil (5-FU) on the first day and 65 mg/kg of 5-FU on the third day. The tip of an 18-gauge needle was used in order to develop a superficial scratching on the left cheek pouch mucosa by dragging twice in a linear movement on third and fifth days. After ulcerative mucositis were clinically detected on the animals' left cheek pouch mucosa, the laser therapy was started. Four different laser wavelengths (660 nm, HELBO, Bredent; 810 nm, Fotona XD, Fotona; 980 nm, ARC Fox; and 1,064 nm, Fidelis Plus 3, Fotona) used for LLLT at ED 8 J/cm2 daily from the first to the fourth days. Oval excisional biopsy was taken from the site of the wound, and the expression of PDGF, TGF-β, and bFGF was evaluated. The obtained data were analyzed by one2-way ANOVA, and then Tukey HSD tests were used for pairwise comparisons among groups (α = 0.05). The one-way ANOVA test indicated that expression values of the growth factors, PDGF and bFGF, were significantly affected by irradiation of different wavelengths of lasers (p < 0.001). However, expression value of the TGF-β was not affected by irradiation of different wavelengths of lasers (p > 0.05). The highest PDGF expression was detected in neodymium-doped yttrium aluminum garnet (Nd:YAG) laser group (p < 0.05), and there were no statistically significant differences among the other groups (p > 0.05). The highest bFGF expression was detected in 980-nm diode and Nd:YAG laser groups (p < 0.05), and there were no statistically significant differences among the other groups (p > 0.05). These findings suggest that low-level Nd:YAG and 980-nm diode laser therapy accelerate the wound healing process by changing the expression of PDGF and bFGF genes responsible for the stimulation of the cell proliferation and fibroblast growth.

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Acknowledgments

The author wishes to express their sincere appreciation to ARC Fox and HELBO companies for supplying the laser devices for this study and to Jan Tunér and René-Jean Bensadoun for sharing their knowledge and experiences about LLLT.

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Authors and Affiliations

  1. Department of Prosthodontics, Bezmialem Vakif University, Vatan Street, Istanbul, Turkey

    Aslihan Usumez

  2. Department of Physiology, Gaziantep University, Gaziantep, Turkey

    Beyhan Cengiz & Tuncer Demir

  3. Department of Medical Biology, Gaziantep University, Gaziantep, Turkey

    Serdar Oztuzcu

  4. Department of Oral Surgery, Gaziantep University, Gaziantep, Turkey

    Mutan Hamdi Aras

  5. Department of Restorative Dentistry, RWTH Aachen University, Aachen, Germany

    Norbert Gutknecht

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  1. Aslihan Usumez
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  2. Beyhan Cengiz
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  3. Serdar Oztuzcu
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  4. Tuncer Demir
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  5. Mutan Hamdi Aras
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  6. Norbert Gutknecht
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Correspondence to Aslihan Usumez.

Additional information

This manuscript is based on the Master Thesis in “Lasers in Dentistry” in RWTH Aachen University.

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Usumez, A., Cengiz, B., Oztuzcu, S. et al. Effects of laser irradiation at different wavelengths (660, 810, 980, and 1,064 nm) on mucositis in an animal model of wound healing. Lasers Med Sci 29, 1807–1813 (2014). https://doi.org/10.1007/s10103-013-1336-z

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  • DOI: https://doi.org/10.1007/s10103-013-1336-z

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