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Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats

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Abstract

Recent studies have demonstrated the efficacy of coherent light therapy from the red region of the electromagnetic spectrum on the tissue-healing process. This study analysed the effect of non-coherent light therapy (light-emitting diode—LED) with or without silver sulfadiazine (sulpha) on the healing process of third-degree burns. In this study, 72 rats with third-degree burns were randomly divided into six groups (n = 12): Gr1 (control), Gr2 (non-contact LED), Gr3 (contact LED), Gr4 (sulfadiazine), Gr5 (sulfadiazine + non-contact LED) and Gr6 (sulfadiazine + contact LED). The groups treated with LED therapy received treatment every 48 h (λ = 640 ± 20 nm, 110 mW, 16 J/cm2; 41 s with contact and 680 s without contact). The digital photometric and histomorphometric analyses were conducted after the burn occurred. The combination of sulpha and LED (contact or non-contact) improved the healing of burn wounds. These results demonstrate that the combination of silver sulfadiazine with LED therapy (λ = 640 ± 20 nm, 4 J/cm2, without contact) improves healing of third-degree burn wounds, significantly reduces the lesion area and increases the granulation tissue, increases the number of fibroblasts, promotes collagen synthesis and prevents burn infections by accelerating recovery.

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Acknowledgments

R. A. Nicolau thanks the National Council for Scientific and Technological Development (CNPq, Brazil) for the productivity fellowship (process no. 314455/2009-4).

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Authors and Affiliations

  1. Faculdade Integral Diferencial—FACID, Rua Veterinário Bugyja Brito, 1354—Bairro Horto Florestal, 64052-410, Teresina, Piauí, Brazil

    Silvana Maria Véras Neves & Antônio Luiz Martins Maia Filho

  2. Universidade do Vale do Paraíba, São José dos Campos, São Paulo, Brazil

    Silvana Maria Véras Neves, Renata Amadei Nicolau & Antônio Luiz Martins Maia Filho

  3. Universidade Federal do Piauí, Teresina, Piaui, Brazil

    Lianna Martha Soares Mendes

  4. Pathology and Cytopathology Laboratory, São Marcos Hospital, Teresina, Piaui, Brazil

    Ana Maria Veloso

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  1. Silvana Maria Véras Neves
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  2. Renata Amadei Nicolau
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Correspondence to Silvana Maria Véras Neves.

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Neves, S.M.V., Nicolau, R.A., Filho, A.L.M.M. et al. Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats. Lasers Med Sci 29, 203–212 (2014). https://doi.org/10.1007/s10103-013-1312-7

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  • DOI: https://doi.org/10.1007/s10103-013-1312-7

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