Skip to main content
SpringerLink
Log in

The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion

  • Original Article
  • Published:
Lasers in Medical Science Aims and scope Submit manuscript

Abstract

The objective of this study was to assess the effects of 780-nm low-level laser therapy at different periods of 7, 14 and 21 days after cryolesion, including the dose (10 or 50 J/cm2), to promote a better muscle repair evidenced by histopathological and immunohistochemical analyses. Fifty-four male rats were divided into three groups: injured control group (CG)—injured animals without any treatment; injured 780-nm laser-treated group, at 10 J/cm2 (G10); and injured 780-nm laser-treated group, at 50 J/cm2 (G50). Each group was divided into three subgroups (n = 6): 7, 14 and 21 days post-injury. Histopathological findings revealed better organised muscle fibres in the G10 and G50 during the periods of 7 and 14 days compared to the CG. The G10 and G50 during the 7 days showed a significant reduction (p < 0.05) of lesion area compared to the CG, without differences between groups treated for 14 and 21 days. The G10 showed an increase of the amount of vessels after 14 days compared to the G50, but not in relation to controls. With regard to the immunohistochemical analyses of the MyoD factor, the G10 and G50 during the 7 days showed higher concentrations of immunomarkers than controls. Myogenin immunomarkers were similarly observed at days 7 and 14 in all the three groups analysed, whereas immunomarkers were found in none of the groups after 21 days of laser therapy. The results showed that laser, regardless the applied dose, has positive effects on muscle repair.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Dawood MS, Al-Salihi AR, Qasim AW (2012) Laser therapy of muscle injuries. Lasers Med Sci. doi:10.1007/s10103-012-1131-2

  2. Huard J, Li Y, Fu F (2002) Muscle injury and repair: current trends in research. J Bone Joint Surg Am 84:822–832

    PubMed  Google Scholar 

  3. Cressoni MD, Dib Giusti HH, Casarotto RA, Anaruma CA (2008) The effects of a 785-nm AlGaInP laser on the regeneration of rat anterior tibialis muscle after surgically-induced injury. Photomed Laser Surg 26:461–466

    Article  Google Scholar 

  4. Amaral CA, Parizotto NA, Salvini TF (2001) Dose-dependency of low-energy HeNe laser effect in regeneration of skeletal muscle in mice. Lasers Med Sci 16:44–51

    Article  CAS  PubMed  Google Scholar 

  5. Baptista J, Martins MD, Pavesi VC, Bussadori SK, Fernandes KP, Júnior DD, Ferrari RA (2011) Influence of laser photobiomodulation on collagen IV during skeletal muscle tissue remodeling after injury in rats. Photomed Laser Surg 29:11–17

    Article  CAS  PubMed  Google Scholar 

  6. Iyomasa DM, Garavelo I, Iyomasa MM, Watanabe I, Issa JPM (2009) Ultrastructural analysis of the low level laser therapy effects on the lesioned anterior tibial muscle in gerbil. Micron 40:413–418

    Article  CAS  PubMed  Google Scholar 

  7. Rizzi EC, Issa JPM, Dias FJ, Leão JC, Regalo SCH, Siéssere S, Watanabe I, Iyomasa MM (2010) Low-level laser intensity application in masseter muscle for treatment purposes. Photomed Laser Surg 28:31–35

    Article  Google Scholar 

  8. Weiss N, Oron U (1992) Enhancement of muscle regeneration in the rat gastrocnemius by low energy laser irradiation. Anat Embryol 186:497–503

    Article  CAS  PubMed  Google Scholar 

  9. Miyabara EH, Martin JL, Griffin TM, Moriscot AS, Mestril R (2006) Overexpression of inducible 70-kDa heat shock protein in mouse attenuates skeletal muscle damage induced by cryolesioning. Am J Physiol Cell Physiol 290:1128–1138

    Article  Google Scholar 

  10. Mesquita-Ferrari RA, Martins MD, Silva JA Jr, Silva TD, Piovesan RF, Pavesi VCS, Bussadori SK, Fernandes KPS (2011) Effects of low-level laser therapy on expression of TNF-α and TGF-β in skeletal muscle during the repair process. Lasers Med Sci 26:335–340

    Article  PubMed  Google Scholar 

  11. Renno AC, Toma RL, Feitosa SM, Fernandes K, Bossini PS, de Oliveira P, Parizotto N, Ribeiro DA (2011) Comparative effects of low-intensity pulsed ultrasound and low-level laser therapy on injured skeletal muscle. Photomed Laser Surg 29:5–10

    Article  PubMed  Google Scholar 

  12. Li J, Chen J, Kirsner R (2007) Pathophysiology of acute wound healing. Clin Dermatol 25:9–18

    Article  CAS  PubMed  Google Scholar 

  13. Silva LH, Silva MT, Gutierrez RM, Conte TC, Toledo CA, Aoki MS, Liebano RE, Miyabara EH (2011) GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin. Lasers Med Sci 27(5):993–1000. doi:10.1007/s10103-011-1031

    Article  PubMed  Google Scholar 

  14. Santos DR, Liebano RE, Baldan CS, Masson IB, Soares RP, Esteves Junior I (2010) The low-level laser therapy on muscle injury recovery: literature review. J Health Sci Inst 28:286–288

    Google Scholar 

  15. Nakano J, Kataoka H, Sakamoto J, Origuchi T, Okita M, Yoshimura T (2009) Low-level laser irradiation promotes the recovery of atrophied gastrocnemius skeletal muscle in rats. Exp Physiol 94:1005–1015

    Article  CAS  PubMed  Google Scholar 

  16. Zhang BT, Yeung SS, Liu Y, Wang HH, Wan YM, Ling SK, Zhang HY, Li YH, Yeung EW (2010) The effects of low frequency electrical stimulation on satellite cell activity in rat skeletal muscle during hindlimb suspension. BMC Cell Biology 11:87

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. McKay BR, O’Reilly CE, Phillips SM, Tarnopolsky MA, Parise G (2008) Coexpression of IGF-1 family members with myogenic regulatory factors following acute damaging muscle lengthening contractions in humans. J Physiol 586:5549–5560

    Article  CAS  PubMed  Google Scholar 

  18. Souza RWA, Gonçalves W, Cavalcante WLG, Pai-Silva MD, Gallacci M (2010) Nandrolone stimulates MyoD expression during muscle regeneration in the condition of myonecrosis induced by Bothrops jararacussu venom poisoning. Journal of Toxicology and Environmental Health, Part A: Current Issues 73(13–14):934–943

    Article  Google Scholar 

  19. Mastroyiannopoulos NP, Nicolaou P, Anayasa M, Uney JB, Phylactou LA (2012) Down-regulation of myogenin can reverse terminal muscle cell differentiation. PLoS One 7:e29896

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Füchtbauer EM, Westphal H (1992) MyoD and myogenin are coexpressed in regenerating skeletal muscle of the mouse. Dev Dyn 193:34–39

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the CAPES for the support provided. The authors thank Rogéria Inês Rosa Lara for her help during data analysis.

Author information

Authors and Affiliations

  1. Post Graduation Program of Surgery, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, Brazil

    Roberta M. Brunelli & Alberto Cliquet Jr.

  2. Department of Physiotherapy, Federal University of São Carlos (UFSCar), São Carlos, São Paulo, Brazil

    Natalia C. Rodrigues & Nivaldo A. Parizotto

  3. Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, São Paulo, Brazil

    Daniel A. Ribeiro, Kelly Fernandes, Angela Magri, Lívia Assis & Ana Claudia M. Renno

  4. Department of Biomechanics, Medicine and Rehabilitation of Locomotor System, School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900, Ribeirão Preto, São Paulo, Brazil, 14049-900

    Daniela C. C. Abreu

Authors
  1. Roberta M. Brunelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Natalia C. Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniel A. Ribeiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kelly Fernandes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Angela Magri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lívia Assis
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nivaldo A. Parizotto
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Alberto Cliquet Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Ana Claudia M. Renno
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Daniela C. C. Abreu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Daniela C. C. Abreu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brunelli, R.M., Rodrigues, N.C., Ribeiro, D.A. et al. The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion. Lasers Med Sci 29, 91–96 (2014). https://doi.org/10.1007/s10103-013-1277-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10103-013-1277-6

Keywords

Search

Navigation