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Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy

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Abstract

The aim of this study was to histologically and histometrically evaluate the influence of repeated adjunctive antimicrobial photodynamic therapy (aPDT) on bone loss (BL) in furcation areas in rats. Periodontitis was induced by placing a ligature around the mandibular molar in 75 rats. The animals were divided into five groups: the SS group was treated with saline solution (SS); the SRP group received scaling and root planing (SRP); the aPDT1 group received SRP as well as toluidine blue (TBO) and low-level laser therapy (LLLT; InGaAlP, 660 nm; 4.94 J/cm2/point) postoperatively at 0 h; the aPDT2 group received SRP as well as TBO and LLLT postoperatively at 0, 24, 28, and 72 h; and the aPDT3 group received SRP, TBO, and LLLT postoperatively at 0, 48, 96, and 144 h. The area of BL in the furcation region of the molar was histometrically analyzed. Data were analyzed statistically (P < 0.05). Animals treated with a single episode of aPDT showed less BL at days 7 and 30 than those who received only SRP treatment. No significant differences were found among the aPDT groups (P > 0.05). Repeated aPDT did not improve BL reduction when compared to a single episode of aPDT.

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Acknowledgments

This study was conducted at the Department of Periodontology, Araçatuba Dental School, State University of São Paulo (UNESP) Araçatuba, São Paulo, Brazil. Mariéllen Longo received a scholarship from the São Paulo State Foundation for Research (FAPESP 2008/10821-3).

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Authors and Affiliations

  1. GEPLO—Group of Study and Research of Lasers in Dentistry, Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University (UNESP), Araçatuba, São Paulo, Brazil

    Valdir Gouveia Garcia, Mariéllen Longo, Leandro Araújo Fernandes, Erivan Clementino Gualberto Junior, Carolina dos Santos Santinoni, Álvaro Francisco Bosco, Maria José Hitomi Nagata & Letícia Helena Theodoro

  2. Department of Periodontology, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos, SP, Brazil

    Valdir Gouveia Garcia

  3. Department of Clinical and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil

    Leandro Araújo Fernandes

  4. Faculdade de Odontologia de Araçatuba-UNESP, Rua Jose Bonifácio 1193, Centro, CEP: 16050-300, Araçatuba, SP, Brazil

    Valdir Gouveia Garcia

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  1. Valdir Gouveia Garcia
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  2. Mariéllen Longo
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  3. Leandro Araújo Fernandes
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  4. Erivan Clementino Gualberto Junior
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  5. Carolina dos Santos Santinoni
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  6. Álvaro Francisco Bosco
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  7. Maria José Hitomi Nagata
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  8. Letícia Helena Theodoro
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Correspondence to Valdir Gouveia Garcia.

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Garcia, V.G., Longo, M., Fernandes, L.A. et al. Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy. Lasers Med Sci 28, 143–150 (2013). https://doi.org/10.1007/s10103-012-1099-y

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  • DOI: https://doi.org/10.1007/s10103-012-1099-y

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