Abstract
The authors tested the diagnostic potential of the portable autofluorescence optical system that was developed in the preoperative evaluation of resection margins, and thus of the resection line safety in patients with low-positioned colorectal carcinoma. A total of 217 spectral measurements of the fluorescence properties of normal (117) and malignant (100) tissues in 19 patients with colorectal carcinoma were accomplished. The measured spectra thus acquired were then evaluated using logistic regression. Using the principal component method, the authors selected the 30 and 40 most significant wavelengths, respectively, which they then used to construct the logistic model. The model met the basic criteria of statistical significance. The classification power of the model was 79.7% (for 30 wavelengths) and 82.5% (for 40). Statistical discrimination was 0.88 and 0.91, respectively. These results confirm that the optical setup that we selected is suitable for the peroperative testing of the distal resection line. It is capable of differentiating with 90% confidence pathological tissue and thus of reliably guiding further histological processing.
Similar content being viewed by others
References
Becker HD, Hohenberger W, Junginger T, Schlang PM et al. (2002) Significance of the patologico-anatomical examinations for surgical oncology. In: Chirurgische Onkologie 1st edn. Georg Thieme Verlag 21–28
Ferreira D, Henriques M, Oliviera R, Correia J, Minas G (2009) Autofluorescence Spectroscopy of a Human Gastrointestinal Carcinom Cell Line. In: Biodevices 2009 - International Conference on Biomedical Electronics and Devices, Porto, 61–66
Banerjee B, Miedema B, Chandrasekhar H (1998) Emission spectra of colonic tissue and endogenous fluorophores. Am J Med Sci 316(3):220–226
Ramanujam N (2000) Fluorescence spectroscopy of neoplastic and non-neoplastic tissues. Neoplasia 2(1–2):89–117
Mayinger B, Jordan M, Horner P, Gerlach C, Muehldorfer S, Bittorf BR, Matzel KE, Hohenberger W, Hahn EG, Guenther K (2003) Endoscopic light-induced autofluorescence spectroscopy for the diagnosis of colorectal cancer and adenoma. J Photochem Photobiol B 70:13–20
Wagnieres GA, Star MW, Wilson CB (1998) In vivo fluorescence spectroscopy and imaging for oncological applications. Photochem Photobiol 68(5):603–632
DaCosta R, Wilson B, Marcon N (2006) Spectroscopy and fluorescence in esophageal diseases. Best Pract Res Clin Gastroenterol 20(1):41–57
Bu-Hong L, Shu-Sen X (2005) Autofluorescence excitation-emission matrices for diagnosis of colonic cancer. World J Gastroenterol 11(25):3931–3934
Zavadil J, Ležal D, Horák L, Ducháč V, Procházka M (2005) Autofluorescence spectroscopy of malignant tissue. JOAM 7(2):1023–1028
Ozaky Y, Czarnile-Matusewicz B, Šašic S (2001) Two-Dimensional Correlation Spectroscopy in Analytical Chemistry. Analytical Sciences, 2001, 17(ICAS2001), Supplement: i663–i666.
Dluhy R, Shanmukh S, Morita S (2006) The application of two-dimensional correlation spectroscopy to surface and interfacial analysis. Surface and Interface Analysis. 2006, 38, 1481–1489.
Tape TG. The Area Under an ROC Curve[online], University of Nebraska Medical Center. In: Interpreting Diagnostic Tests, University of Nebraska Medical Center. Available at: HYPERLINK "http://darwin.unmc.edu/dxtests/roc3.htm" http://darwin.unmc.edu/dxtests/roc3.htm
Becker HD, Hohenberger W, Junginger T, Schlang PM et al. (2002) Rectal Carcinoma. In: Chirurgische Onkologie 1st edn. Georg Thieme Verlag, pp 505–545
Wheeler JMD, Warren BF, Jones AC, Mortensen NJM (1999) Preoperative radiotherapy for rectal cancer: implications for surgeons, pathologists and radiologists. Br J Surg 86(9):1108–1120
Ueda Y, Kobayashi M (2004) Spectroscopic studies of autofluorescence substances existing in humat tissue: influences of lactic acid and porphyrins. Applied Optics 43(20):3993–3998
Tuchin VV (2003) Optical properties of tissue. In: Vo-Dinh T (ed), Biomedica Photonic Handbook (1st edn). CRC Press
Vo-Dinh T (2003) Basic instrumentation in photonics. In: Biomedical Photonic Handbook (1st edn). CRC Press
Horak L, Zavadil J, Duchac V, Javorsky S, Kostka F, Svec A, Lezal D (2006) Auto-fluorescence spectroscopy of colorectal carcinoma: ex vivo study. JOAM 8(1):396–399
Huang Z, Zheng W, Xie S, Chen R, Zeng H, McLean DI, Lui H (2004) Laser-induced autofluorescence microscopy of normal and tumor human colonic tissue. Int J Oncol 24:59–63
Acknowledgements
We greatly appreciate the cooperation of S. Javorsky (*) who helped set up the fiber-optic system.
* Institute of Photonics and Electronics, AS CR
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ducháč, V., Zavadil, J., Vránová, J. et al. Peroperative optical autofluorescence biopsy—verification of its diagnostic potential. Lasers Med Sci 26, 325–333 (2011). https://doi.org/10.1007/s10103-010-0847-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10103-010-0847-0