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Preclinical ex vivo evaluation of the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses

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Abstract

Objectives

To assess the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses in freshly removed surgical specimens, in preparation for its clinical development.

Methods

Sixty-four breast masses from consenting patients who had undergone either a lumpectomy or a mastectomy were included. Label-free fluorescence spectral acquisitions were obtained with a 25G fibre-containing needle inserted into the mass. Data from benign and malignant masses were compared to establish the most discriminating thresholds and measurement algorithms. Accuracy was verified using the bootstrap method.

Results

The final histological examination revealed 44 invasive carcinomas and 20 benign lesions. The maximum intensity of fluorescence signal was discriminant between benign and malignant masses (p < .0001) whatever their sizes. Statistical analysis indicated that choosing five random measurements per mass was the best compromise to obtain high sensitivity and high negative predictive value with the fewest measurements. Thus, malignant tumours were identified with a mean sensitivity, specificity, negative and positive predictive value of 98.8%, 85.4%, 97.2% and 93.5%, respectively.

Conclusion

This new in situ tissue autofluorescence evaluation device allows accurate discrimination between benign and malignant breast masses and deserves clinical development.

Key Points

• A new device allows in situ label-free fluorescence analysis of ex vivo breast masses

• Maximum fluorescence intensity discriminates benign from malignant masses (p < .0001)

• Five random measurements allow a high negative predictive value (97.2%)

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Acknowledgements

We would like to thank the various biological resource centres for providing the paraffin blocks required for a centralized histological review.

We would also like to thank Lionel Auffray from the Fluids, Automatic and Thermal Systems Laboratory for his engineering expertise, particularly in high-quality 3D printing and Yuki Takahashi for editing this manuscript.

Funding

This study has received funding by Nodea Medical.

Author information

Author notes

  1. Marie-Christine Mathieu and Alexis Toullec contributed equally to this work.

Authors and Affiliations

  1. Gustave Roussy, 114 rue Edouard Vaillant, 94805, Villejuif, France

    Marie-Christine Mathieu, Pierre Maroun, Philippe Vielh, Suzette Delaloge & Corinne Balleyguier

  2. Institut des Sciences Moléculaires d’Orsay (ISMO), CNRS, Univ. Paris-Sud, Université Paris-Saclay, F-91405, Orsay, France

    Alexis Toullec, Lama Alchab & Marie-Pierre Fontaine-Aupart

  3. Nodea Medical, 1 mail du Pr Georges Mathé, 94800, Villejuif, France

    Charlotte Benoit, Pauline Beaumel & Hélène Moniz-Koum

  4. Institut Mutualiste Montsouris, 42 Boulevard Jourdan, 75014, Paris, France

    Richard Berry & Pierre Validire

  5. Hôpital Privé d’Antony, 1 Rue Velpeau, 92160, Antony, France

    Yves Vincent

  6. Laboratoire Aimé Cotton, Université Paris-Sud, ENS Cachan, CNRS, Université Paris-Saclay, 91405, Orsay Cedex, France

    René Farcy

Authors
  1. Marie-Christine Mathieu
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  2. Alexis Toullec
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  3. Charlotte Benoit
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  4. Richard Berry
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  5. Pierre Validire
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  7. Yves Vincent
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  8. Pierre Maroun
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  9. Philippe Vielh
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  10. Lama Alchab
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  12. Hélène Moniz-Koum
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  13. Marie-Pierre Fontaine-Aupart
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  14. Suzette Delaloge
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  15. Corinne Balleyguier
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Corresponding author

Correspondence to Marie-Christine Mathieu.

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Guarantor

The scientific guarantor of this publication is MC Mathieu, MD

Conflict of interest

The authors of this manuscript declare relationships with the following companies: Nodea Medical.

Statistics and biometry

MedPass International (France) kindly provided statistical analysis for this manuscript.

Informed consent

Written informed consent was obtained from all subjects (patients) in this study.

Ethical approval

Institutional review board approval was obtained.

Methodology

• prospective

• observational

• multicentre study

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Cite this article

Mathieu, MC., Toullec, A., Benoit, C. et al. Preclinical ex vivo evaluation of the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses. Eur Radiol 28, 2507–2515 (2018). https://doi.org/10.1007/s00330-017-5228-7

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  • DOI: https://doi.org/10.1007/s00330-017-5228-7

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