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Survival and Growth of Burkholderia cepacia Within the Free-Living Amoeba Acanthamoeba polyphaga

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Abstract

 The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.

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Authors and Affiliations

  1. Leeds Public Health Laboratory, Leeds LS15 7TR, UK, , , , , , GB

    T. J. Rowbotham

  2. Department of Microbiology, The General Infirmary at Leeds, Leeds LS1 3EX, UK, , , , , , GB

    M. Denton

  3. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, , , , , , GB

    P. Landers, K. G. Kerr, J. L. Tipper, P. M. Keig & E. Ingham

Authors
  1. P. Landers
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  2. K. G. Kerr
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  3. T. J. Rowbotham
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  4. J. L. Tipper
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  5. P. M. Keig
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  6. E. Ingham
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  7. M. Denton
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Landers, P., Kerr, K., Rowbotham, T. et al. Survival and Growth of Burkholderia cepacia Within the Free-Living Amoeba Acanthamoeba polyphaga . EJCMID 19, 121–123 (2000). https://doi.org/10.1007/s100960050442

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  • DOI: https://doi.org/10.1007/s100960050442

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