Abstract
A series of 35 strains of Candida glabrata isolated from 29 subjects (5 AIDS patients and 24 HIV-seronegative individuals) were typed by electrophoretic karyotyping and tested for their susceptibilities to both fluconazole and itraconazole. Almost every individual harboured his/her own specific isolate (DNA type). Neither the source of isolation nor the patient's HIV status was associated with a given DNA type. Recurrences were generally due to the persistence of the same DNA type over time. Only 9% of the isolates showed reduced susceptibility to fluconazole (MIC≥8.0 μg/ml), while 43% of the isolates showed reduced susceptibility to itraconazole (MIC≥0.25 μg/ml) (P=0.02). These data show that electrophoretic karyotyping is a useful technique for DNA typing of isolates of Candida glabrata. Care must be taken prior to inititation of antifungal therapy with either of these drugs.
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Barchiesi, F., Falconi Di Francesco, L., Arzeni, D. et al. Electrophoretic Karyotyping and Triazole Susceptibility of Candida glabrata Clinical Isolates. EJCMID 18, 184–187 (1999). https://doi.org/10.1007/s100960050255
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DOI: https://doi.org/10.1007/s100960050255