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Strain variation and antigenic divergence among Bordetella pertussis circulating strains isolated from patients in Iran

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Abstract

Despite global efforts and widespread vaccination to control whooping cough (pertussis) caused by B. pertussis, the re-emergence of pertussis still is being reported all over the world. Antigenic divergence in B. pertussis virulence factors is one of the reasons of pertussis resurgence, resulting in dissimilarity of local and vaccine strains. In this study, clonal spread and variation of B. pertussis virulence factor in isolated strains from Iranian patients have been analyzed. A total of 100 B. pertussis isolates were obtained from Pertussis Reference Laboratory of Pasteur Institute of Iran. Real-time PCR were performed to confirm the B. pertussis strains. The genomic patterns of B. pertussis strains were analyzed by pulsed-field gel electrophoresis (PFGE). Predominant alleles of local strains were ptxP3, ptxA1, prn2, fim 2-1, fim3-2, and cya2. PFGE results showed 25 patterns clustered into 18 PFGE groups. A few similarities between the circulating isolates, vaccine, and standard strains were obtained. Significantly, 48% of the isolates showed dominant pattern with different allelic profiles from vaccine strains. According to the genomic profiles, the clonal spread was observed among the circulating strains. Predominant virulence factor profile was also comparable with other countries. It may be suggested that strain variation between vaccine and local strains may have an effect on pertussis resurgence in Iran like other parts of the world.

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Acknowledgments

We would also like to show our gratitude to all the staff in Pertussis Reference Laboratory‚ Department of Bacteriology and Microbiology Research Center who shared their wisdom with us during this research.

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Authors and Affiliations

  1. Pertussis Reference Laboratory‚ Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran

    Fatemah Sadeghpour Heravi, Vajihe Sadat Nikbin, Masomeh Nakhost Lotfi, Pouran Badiri, Nazanin Jannesar Ahmadi, Seyed Mohsen Zahraei & Fereshteh Shahcheraghi

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  1. Fatemah Sadeghpour Heravi
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  2. Vajihe Sadat Nikbin
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  3. Masomeh Nakhost Lotfi
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  4. Pouran Badiri
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  6. Seyed Mohsen Zahraei
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  7. Fereshteh Shahcheraghi
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Correspondence to Fereshteh Shahcheraghi.

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Fatemah Sadeghpour Heravi and Vajihe Sadat Nikbin have equally collaborated and should be considered co-first authors.

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Sadeghpour Heravi, F., Nikbin, V.S., Nakhost Lotfi, M. et al. Strain variation and antigenic divergence among Bordetella pertussis circulating strains isolated from patients in Iran. Eur J Clin Microbiol Infect Dis 37, 1893–1900 (2018). https://doi.org/10.1007/s10096-018-3323-6

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  • DOI: https://doi.org/10.1007/s10096-018-3323-6

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