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Multi-center evaluation of the VITEK® MS system for mass spectrometric identification of non-Enterobacteriaceae Gram-negative bacilli

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Abstract

Studies have demonstrated that matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid, accurate method for the identification of clinically relevant bacteria. The purpose of this study was to evaluate the performance of the VITEK MS v2.0 system (bioMérieux) for the identification of the non-Enterobacteriaceae Gram-negative bacilli (NEGNB). This multi-center study tested 558 unique NEGNB clinical isolates, representing 18 genera and 33 species. Results obtained with the VITEK MS v2.0 were compared with reference 16S rRNA gene sequencing and when indicated recA sequencing and phenotypic analysis. VITEK MS v2.0 provided an identification for 92.5 % of the NEGNB isolates (516 out of 558). VITEK MS v2.0 correctly identified 90.9 % of NEGNB (507 out of 558), 77.8 % to species level and 13.1 % to genus level with multiple species. There were four isolates (0.7 %) incorrectly identified to genus level and five isolates (0.9 %), with one incorrect identification to species level. The remaining 42 isolates (7.5 %) were either reported as no identification (5.0 %) or called “mixed genera” (2.5 %) since two or more different genera were identified as possible identifications for the test organism. These findings demonstrate that the VITEK MS v2.0 system provides accurate results for the identification of a challenging and diverse group of Gram-negative bacteria.

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Acknowledgements

This study was sponsored by bioMérieux, Durham. NC, USA. We sincerely thank the technical staff at each clinical trial site for their support. We thank David Pincus, Constance Bradford, and Karen MacDonald for providing technical support and statistical analysis.

Transparency declaration

John A. Branda, Jenna A. Rychert, and Mary Jane Farraro have received research funding from bioMérieux and Becton Dickinson and Co. Christine C. Ginocchio has received research funding and/or consulting fees from bioMérieux, Becton Dickinson, Biofire, Biohelix, Nanosphere, Curetis, and Luminex. Gary Procop has received research funding from bioMérieux, Bruker, CDC and Luminex. Sandra S. Richter has received research funding from bioMérieux, Nanosphere, and Forest Laboratories. Carey-Ann Burnham has received research funding from bioMérieux, Accler8, Cepheid, and T2 Biosystems. None of the other authors has any conflicts to disclose.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, North Shore-LIJ Health System Laboratories, 10 Nevada Drive, Lake Success, NY, 11042, USA

    R. Manji, M. Bythrow & C. C. Ginocchio

  2. Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

    J. A. Branda, M. J. Ferraro & J. A. Rychert

  3. Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA

    C.-A. D. Burnham & L. F. Westblade

  4. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    O. B. Garner, M. A. Lewinski & A. B. Mochon

  5. Barnes Jewish Hospital, St. Louis, MO, USA

    R. Jennemann

  6. Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

    G. W. Procop, S. S. Richter & L. Sercia

  7. Department of Pathology and Laboratory Medicine, Hofstra North Shore-LIJ School of Medicine, Hempstead, NY, USA

    L. F. Westblade & C. C. Ginocchio

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  1. R. Manji
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  2. M. Bythrow
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  8. M. A. Lewinski
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Correspondence to C. C. Ginocchio.

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Manji, R., Bythrow, M., Branda, J.A. et al. Multi-center evaluation of the VITEK® MS system for mass spectrometric identification of non-Enterobacteriaceae Gram-negative bacilli. Eur J Clin Microbiol Infect Dis 33, 337–346 (2014). https://doi.org/10.1007/s10096-013-1961-2

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