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A comparison of the Sensititre® MYCOTB panel and the agar proportion method for the susceptibility testing of Mycobacterium tuberculosis

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Abstract

The agar proportion method (APM) for determining Mycobacterium tuberculosis susceptibilities is a qualitative method that requires 21 days in order to produce the results. The Sensititre method allows for a quantitative assessment. Our objective was to compare the accuracy, time to results, and ease of use of the Sensititre method to the APM. 7H10 plates in the APM and 96-well microtiter dry MYCOTB panels containing 12 antibiotics at full dilution ranges in the Sensititre method were inoculated with M. tuberculosis and read for colony growth. Thirty-seven clinical isolates were tested using both methods and 26 challenge strains of blinded susceptibilities were tested using the Sensititre method only. The Sensititre method displayed 99.3% concordance with the APM. The APM provided reliable results on day 21, whereas the Sensititre method displayed consistent results by day 10. The Sensititre method provides a more rapid, quantitative, and efficient method of testing both first- and second-line drugs when compared to the gold standard. It will give clinicians a sense of the degree of susceptibility, thus, guiding the therapeutic decision-making process. Furthermore, the microwell plate format without the need for instrumentation will allow its use in resource-poor settings.

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Acknowledgments

This work was supported by Trek Diagnostic Systems. We would like to thank Michael Tosi, MD, Roberto Posada, MD, and Shirish Huprikar, MD, for their invaluable input and guidance. An abstract of this study was presented at the Pediatric Academic Societies Meeting held on April 30–May 3 2011, Denver, Colorado.

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Authors and Affiliations

  1. Department of Pediatric Infectious Diseases, Mount Sinai School of Medicine, Mount Sinai Medical Center, 1 Gustave L. Levy Place, Box 1657, New York, NY, 10029, USA

    M. M. Abuali & V. J. LaBombardi

  2. Department of Microbiology, Mount Sinai School of Medicine, Mount Sinai Medical Center, New York, NY, USA

    R. Katariwala & V. J. LaBombardi

Authors
  1. M. M. Abuali
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  2. R. Katariwala
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  3. V. J. LaBombardi
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Correspondence to M. M. Abuali.

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Abuali, M.M., Katariwala, R. & LaBombardi, V.J. A comparison of the Sensititre® MYCOTB panel and the agar proportion method for the susceptibility testing of Mycobacterium tuberculosis . Eur J Clin Microbiol Infect Dis 31, 835–839 (2012). https://doi.org/10.1007/s10096-011-1382-z

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  • DOI: https://doi.org/10.1007/s10096-011-1382-z

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