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Comparison of an in-house real-time RT-PCR assay with a commercial assay for detection of enterovirus RNA in clinical samples

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Abstract

Molecular detection of enterovirus (EV) RNA based on PCR methods is a quicker and more sensitive approach than culture methods. At present, different PCR-based methods for EV RNA detection are available, but comparisons of results obtained according to the different approaches are limited. We evaluated an in-house real-time RT-PCR assay with a commercialized TaqMan real-time RT-PCR kit for detection of EV. Consecutive clinical specimens from paediatric patients less than 6 years old with clinical suspicion of EV infection were analyzed between July and November 2010. After RNA extraction, samples were amplified both by the real-time RT-PCR commercial assay and the in-house assay. A total of 19 of 132 patients (14.4%) involving 20 samples (14 plasma samples and 6 CSF) were positive in at least one of the two assays. The sensitivity of the in-house assay when the MutaPLATE® assay was used as a reference was 90% (IC 95%; 74.35–100) and the specificity was 100% (IC 95%; 99.63–100). Cts results of two methods were statistically correlated (r = 0.774; P = 0.01). In conclusion, these two real-time RT-PCR assays are rapid and easy methods for detection of EV.

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Acknowledgments

This study was partially supported by Agència d'Avaluació de Tecnologia i Recerca Mèdiques (AATRM) and AGAUR 2009/SGR00136.

We thanks Nuria Cabrerizo for technical support.

Potential conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Molecular Microbiology, \Hospital Universitari Sant Joan de Deu, Esplugues, Barcelona, Spain

    L. Selva & C. Muñoz-Almagro

  2. Department of Paediatrics, Hospital Universitari Sant Joan de Deu, Esplugues, Barcelona, Spain

    A. Martinez-Planas & J.-J. García-García

  3. Department of Emergency Care, Hospital Universitari Sant Joan de Deu, Esplugues, Barcelona, Spain

    R. Casadevall & C. Luaces

  4. Molecular Microbiology Department, Hospital Sant Joan de Déu, University of Barcelona, P° Sant Joan de Déu n° 2 , 08950, Esplugues, Barcelona, Spain

    C. Muñoz-Almagro

Authors
  1. L. Selva
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  2. A. Martinez-Planas
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  3. J.-J. García-García
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  4. R. Casadevall
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  5. C. Luaces
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  6. C. Muñoz-Almagro
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Corresponding author

Correspondence to C. Muñoz-Almagro.

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Selva, L., Martinez-Planas, A., García-García, JJ. et al. Comparison of an in-house real-time RT-PCR assay with a commercial assay for detection of enterovirus RNA in clinical samples. Eur J Clin Microbiol Infect Dis 31, 715–719 (2012). https://doi.org/10.1007/s10096-011-1364-1

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  • DOI: https://doi.org/10.1007/s10096-011-1364-1

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