Skip to main content

Advertisement

SpringerLink
Log in

Molecular typing of Candida albicans strains isolated from denture wearers by repetitive sequence-based PCR

  • Article
  • Published:
European Journal of Clinical Microbiology & Infectious Diseases Aims and scope Submit manuscript

Abstract

Long-term use of prosthesis is the most important risk factor for the colonization of Candida species on the mucosal surfaces, which can lead to the development of denture-related stomatitis (DRS). Some individuals wearing prosthesis develop DRS and others do not. C. albicans strains isolated from both groups were genotypically compared. The purpose of this study was to determine whether the strain causing prosthesis stomatitis was different from the other strains genotypically. The study included 90 individuals wearing different prostheses and 20 control individuals with natural teeth. In the study 109 C. albicans strains were used which were isolated from the saliva samples and the mucosal surfaces of the tongues and palates of 51 individuals and then defined phenotypically. Phenotypic diagnosis of the isolates was genotypically verified by using species-specific PCR. For molecular typing, repetitive extragenic palindromic sequence polymerase chain reaction (REP-PCR) was employed. The results of the study revealed that REP-PCR had the capability to separate 109 C. albicans strains and six reference strains into 44 genotypes. Whereas C. albicans strains showed heterogenic distribution, C. albicans strains isolated from the individuals suffering from prosthesis stomatitis showed no specific genotypes. REP-PCR is a simple, fast and low-cost method and helped work on a great number of samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Budtz-Jorgensen E (2000) Ecology of Candida-associated denture stomatitis. Microb Ecol Health Dis 12:170–185

    Article  Google Scholar 

  2. Darwazeh AMG, Al-Refai S, Al-Mojawel S (2001) Isolation of Candida species from the oral cavity and fingertips of complete denture wearers. J Proshet Dent 86:420–423

    Article  CAS  Google Scholar 

  3. Pires FR, Santos EBD, Bonan PR, Almeida OP (2002) Denture stomatitis and salivary Candida in Brazilian edentulous patients. J Oral Rehabil 29:1115–1119

    Article  CAS  PubMed  Google Scholar 

  4. Samaranayake LP, Cheung LK, Samaranayake YT (2002) Candidiasis and other fungal diseases of the mouth. Dermatol Ther 15:251–269

    Article  Google Scholar 

  5. Soll DR (2000) The ins and outs of DNA fingerprinting the infectious fungi. Clin Microbiol Rev 13:332–370

    Article  CAS  PubMed  Google Scholar 

  6. Dassanayake RS, Samaranayake LP (2003) Amplification-based nucleic acid scanning techniques to assess genetic polymorphism in Candida. Crit Rev Microbiol 54:249–258

    Google Scholar 

  7. Stachie RF, Healy M (2006) Molecular strain typing using repetitive sequence-based PCR. In: Tang, YW, Strattan, CW (Eds), Advanced techniques in diagnostic microbiology. Springer Science + Business Media, LLC

  8. Olive DM, Pamela B (1999) Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37:1661–1669

    CAS  PubMed  Google Scholar 

  9. Redkar RJ, Dube MP, McCleskey FK, Rinaldi MG, Delvecchio VG (1996) DNA fingerprinting of Candida rugosa via repetitive sequenced-based PCR. J Clin Microbiol 34:1677–1681

    CAS  PubMed  Google Scholar 

  10. Liu D, Coloe S, Baird R, Pederson J (2000) Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol 38:471

    CAS  PubMed  Google Scholar 

  11. Olgun A, Topal A, Temizkan G, Yılmazer S (1998) Methods used in molecular biology. Research and application center of biotechnology and genetic engineering. Publication no. 1, BİYOGEM (in Turkish)

  12. Mannarelli BM, Kurtzman CP (1998) Rapid Identification of Candida albicans and other human pathogenic yeast by using short oligonuclotides in a PCR. J Clin Microbiol 36:1634–1641

    CAS  PubMed  Google Scholar 

  13. Abaci O, Haliki-Uztan A, Ates M (2008) Specific identification of Candida albicans and Candida dubliniensis by PCR using species-specific primers. Ann Microbiol 58(2):325–331

    Article  CAS  Google Scholar 

  14. SPSS 11.0 for Windows. SPSS Inc., Chicago, IL, USA

  15. Abaci O, Haliki-Uztan A, Ozturk B, Toksavul S, Ulusoy M, Boyacioglu H (2010) Determining Candida spp. incidence in denture wearers. Mycopathologia. doi:10.1007/s11046-010-9275-8

    PubMed  Google Scholar 

  16. Senpuku H, Sogame A, Inoshitu E, Tsuha Y, Miyazaki H, Hanada N (2003) Systemic diseases in association with microbial species in oral biofilm from elderly requiring care. Gerontology 49(5):301–309

    Article  CAS  PubMed  Google Scholar 

  17. Reiss E et al (1998) Molecular diagnosis and epidemiology of fungal infections. Med Mycol 36(suppl 1):249–257

    CAS  PubMed  Google Scholar 

  18. Hossain H, Ansari F, Schulz-Weidner N, Wetzel W-E, Chakraborty T, Domann E (2003) Clonal Identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre-school children. Oral Microbiol Immunol 18:302–308

    Article  CAS  PubMed  Google Scholar 

  19. Samaranayeke YH, Samaranayeke LP, Yau JYY, Dassanayake RS, Li TKL, Anil S (2003) Phenotyping diversity of oral C.albicans isolated on single and sequential visits in an HIV-infected Chinese cohort. APMIS 111:329–337

    Article  Google Scholar 

  20. Waltimo TMT, Dassanayake RS, Ørstavik D, Haapasalo MPP, Samaranayeke LP (2001) Phenotypes and randomly amplified polymorphic DNA profiles of Candida albicans isolates from root canal infections in a Finnish population. Oral Microbiol Immunol 16:106–112

    Article  CAS  PubMed  Google Scholar 

  21. Leung KW, Dassanayake RS, Yau JYY, Jin LJ, Yam WC, Samaranayake LP (2000) Oral colonization, phenotypic, and genotypic profiles of Candida species in irradiated dentate, xerostomic nasopharyngeal carcinoma survivors. J Clin Microbiol 38(6):2219–2226

    CAS  PubMed  Google Scholar 

  22. Xu J, Ramos AR, Vilgalys R, Mitchell TG (2000) Clonal and spotaneous origins of fluconazole resistance in Candida albicans. J Clin Microbiol 38:1214–1220

    CAS  PubMed  Google Scholar 

  23. Gil-Lamaignere C, Roilides E, Hacker J, Müller F-MC (2003) Molecular typing for fungi—a critical review of the possibilities and limitations of current and future methods. Clin Microbiol Infect 9:172–185

    Article  CAS  PubMed  Google Scholar 

  24. Cross LJ, Williams DW, Sweeney CP, Jackson MS, Lewis MAO, Bagg J (2004) Evaluation of the recurrence of denture stomatitis and Candida colonization in a small group of patients who received itraconazole. Oral Surg Oral Med Oral Pathol Oral Radiol Edod 97:351–358

    Article  Google Scholar 

  25. Mathaba LT, Davies G, Warmington JR (1995) The genotyping relationship of Candida albicans strains isolated from the oral cavity of patients with denture stomatitis. J Med Microbiol 42:372–379

    Article  CAS  PubMed  Google Scholar 

  26. Pires-Gonçalves RH, Miranda ET, Baeza LC, Matsumoto MT, Zaia JE, Mendes G (2007) Genetic relatedness of commensal strains of Candida albicans carried in the oral cavity of patients’ dental prosthesis users in Brazil. Mycopathologia 164:255–263

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Science, Department of Biology, Basic & Industrial Microbiology Section, Ege University, Bornova-, Izmir, Turkey

    O. Abaci & A. Haliki-Uztan

  2. Faculty of Dentistry, Department of Prosthetic Dentistry, Ege University, Bornova-, Izmir, Turkey

    B. Ozturk, S. Toksavul & M. Ulusoy

  3. Faculty of Science, Department of Statistics, Ege University, 35100, Bornova-Izmir, Turkey

    H. Boyacioglu

Authors
  1. O. Abaci
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Haliki-Uztan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Ozturk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Toksavul
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Ulusoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Boyacioglu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. Abaci.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abaci, O., Haliki-Uztan, A., Ozturk, B. et al. Molecular typing of Candida albicans strains isolated from denture wearers by repetitive sequence-based PCR. Eur J Clin Microbiol Infect Dis 30, 141–149 (2011). https://doi.org/10.1007/s10096-010-1062-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-010-1062-4

Keywords

Search

Navigation