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Genetic relatedness of commensal strains of Candida albicans carried in the oral cavity of patients’ dental prosthesis users in Brazil

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Abstract

The aim of this study is to describe the degree of yeast-colonization in diabetic and hemodialysed-users of dental prostheses. Individuals (306) were examined using an oral rinse technique in order to evaluate the incidence of yeast-carriage, and genotype of C. albicans. Yeasts were isolated from 68.4% (91/133) individual’s dental prostheses users. Dental prostheses were found to be a significant factor for the yeast colonization (P < 0.05). Overall, the intensity of carriage was higher in diabetic patients as compared with health and hemodialysed individuals (P < 0.05). The isolation rates were: C. albicans (51.7%), C. parapsilosis (20.9%), C. tropicalis (14.3%), C. glabrata (6.6%), C. krusei (3.3%), C. rugosa (1.1%), and Pichia (Pichia ohmeri, 2.2%). Ready-To-Go RAPD Analysis Beads were used and primer OPJ 6 distinguished the C. albicans isolates found in prostheses users. All the isolates were grouped into 11 RAPD profiles in four main clusters and, the average S AB for the entire collection of 47 C.␣albicans isolates were 0.779 ± 0.178. Over 85% of isolates had a similarity level higher than or equal to 0.8 reinforcing the idea that the use of dental prostheses, independently of the host’s clinical condition, probably provides the necessary conditions for these strains to gain a growth-specific advantage over others.

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Acknowledgments

This study was supported by CNPq (process 476595/2003). We are most grateful to Dr Aníbal Moysés Simão Júnior, Dr José Vaner Pedigone and Dr Marco Antônio Benedetti Filho and their teams for their contributions to this study.

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Authors and Affiliations

  1. Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, UNESP, Araraquara, SP, Brazil

    Regina Helena Pires-Gonçalves, Elaine Toscano Miranda, Lilian Cristiane Baeza, Marcelo Teruyuki Matsumoto & Maria José Soares Mendes-Giannini

  2. Universidade de Franca, Franca, SP, Brazil

    José Eduardo Zaia

  3. R. Expedicionários do Brasil, CEP 14802-901, Araraquara, SP, 1621, Brazil

    Maria José Soares Mendes-Giannini

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  1. Regina Helena Pires-Gonçalves
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  2. Elaine Toscano Miranda
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  3. Lilian Cristiane Baeza
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  4. Marcelo Teruyuki Matsumoto
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  5. José Eduardo Zaia
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  6. Maria José Soares Mendes-Giannini
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Correspondence to Maria José Soares Mendes-Giannini.

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Pires-Gonçalves, R.H., Miranda, E.T., Baeza, L.C. et al. Genetic relatedness of commensal strains of Candida albicans carried in the oral cavity of patients’ dental prosthesis users in Brazil. Mycopathologia 164, 255–263 (2007). https://doi.org/10.1007/s11046-007-9052-5

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  • DOI: https://doi.org/10.1007/s11046-007-9052-5

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