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Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells

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Abstract

GPR137 are ubiquitously expressed in the central nervous system. However, the role o f GPR137 in human malignant glioma is still poorly known. In the present study, we firstly detected the expression of GPR137 in 29 human glioma tissue specimens by immunohistochemistry and in 5 malignant glioma cell lines by quantitative RT-PCR. The expression of GPR137 was much stronger in high-grade gliomas than in low-grade gliomas. Lentivirus-mediated small interfering RNAs (siRNAs) were employed to knock down GPR137 expression in glioma cells. Inhibition of GPR137 expression by RNAi significantly inhibited the proliferation and colony-forming capacity of U251, A172 and U373 cells. Moreover, flow cytometry analysis showed that knockdown of GPR137 led to the cell-cycle arrest at the S phase. Our results indicated that GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells.

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Acknowledgments

We thank the great help from the Second Affiliated Hospital of Anhui Medical University, and supports from National Natural Science Foundation of China (81072066).

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Authors and Affiliations

  1. Department of Neurosurgery, The Second Affiliated Hospital of Anhui Medical University, and Cerebral Vascular Disease Research Center, Anhui Medical University, 678 Fu Rong Road, Hefei, 230601, China

    Gang Zong, Hongliang Wang, Jia Li, Yongsheng Xie, Erbao Bian & Bing Zhao

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  1. Gang Zong
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  2. Hongliang Wang
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  3. Jia Li
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  4. Yongsheng Xie
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  5. Erbao Bian
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  6. Bing Zhao
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Corresponding author

Correspondence to Bing Zhao.

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Figure S1. Down-regulation of GPR137 inhibited the colony formation of U373 glioma cells. (A) Expression analyses of GPR137 mRNA levels in U373 cells. (B) Representative images of the size and number of colonies in Con, Lv-shCon and Lv-shGPR137 groups under fluorescent microscopy and light microscopy (4× lens). (C) Statistical analysis of the number of colonies with crystal violet staining. There was a significant difference between Lv-shCon and Lv-shGPR137 (**p < 0.01, ***p < 0.001).

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Zong, G., Wang, H., Li, J. et al. Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells. Neurol Sci 35, 1707–1714 (2014). https://doi.org/10.1007/s10072-014-1817-2

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