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Endoplasmic reticulum in the penumbra following middle cerebral artery occlusion in the rabbit

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Abstract

Caspase-12 has been localized to endoplasmic reticulum (ER) and showed to involve ER stress-induced apoptosis. In the present work we investigated the temporospatial alterations of caspase-12 immunoreactivity in the penumbra following cerebral ischemia/reperfusion in rabbit. Transient cerebral ischemia was produced by intraluminal occlusion of the middle cerebral artery for 2 h followed by 1 h, 6 h, 1 day, 3 days, 7 days and 14 days of reperfusion. Caspase-12 immunohistochemistry was first increased in the penumbra 1 h after reperfusion, with a peak at day 1 to day 3, and then gradually decreased to basal level at day 14. The number of TUNEL-positive cells and ultrastructural observation of brain sections in the penumbra showed a similar change at the same time points. ER mediated by caspase-12 participated in apoptosis induced by cerebral ischemia/reperfusion injury, which may provide a new area for therapeutic intervention to ameliorate outcomes following cerebral ischemia.

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Acknowledgments

This study was supported by China Ministry of Health Science Foundation (#200310, Dr. Liu), Hebei Natural Science Foundation (#C2006000927, Dr. Liu). China Postdoctor Science Foundation (#20070411051, Dr. Yang).

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Authors and Affiliations

  1. Department of Medical Imaging, The Second Hospital of Hebei Medical University, 215 West Heping Road, Shijiazhuang, 050000, Hebei Province, China

    Huai-jun Liu, Ji-Ping Yang, Cang-Hai Wang & Ying Li

  2. Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China

    Rui-Chun Liu & Chun-Yan Li

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  1. Huai-jun Liu
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  2. Ji-Ping Yang
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  3. Cang-Hai Wang
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  4. Rui-Chun Liu
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  5. Ying Li
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  6. Chun-Yan Li
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Correspondence to Huai-jun Liu.

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Liu, Hj., Yang, JP., Wang, CH. et al. Endoplasmic reticulum in the penumbra following middle cerebral artery occlusion in the rabbit. Neurol Sci 30, 227–232 (2009). https://doi.org/10.1007/s10072-009-0086-y

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  • DOI: https://doi.org/10.1007/s10072-009-0086-y

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