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Microbial succession in different years of pit mud from a distillery in Sichuan for Nong-xiang Baijiu fermentation

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Abstract

Microbial community and succession of 5-, 20-, and 50-year pit mud (PM) were uncovered in this study. The results showed that Bacteroidetes, Firmicutes and Ascomycota were dominant phyla in these PM samples. Interestingly, most sequences could not be classified into fungal taxa at the genus level by UNITE Database, the diversity and richness of bacteria in these PMs were higher than that of fungi. It was noteworthy that both 20-year and 50-year PMs exhibited higher abundances of Caproiciproducens and Petrimonas when compared with 5-year PM. While higher proportions of Lactobacillus and Acinetobacter were observed in the 5-year PM. Furfermore, these PMs microbiota mainly involved biosynthesis, degradation, and generation of precursor metabolites, which contributed to carbon cycling of Nong-xiang Baijiu anaerobic fermentation. Taken together, lactic acid bacteria depletion and caproic acid bacteria accumulation might be an important succession trend of PM microbiota during the long-term fermentation of Chinese Nong-xiang Baijiu.

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Funding

This work was funded by Sichuan Province Science and Technology Innovation Seedling Project (MZGC20230121), Doctoral Scientific Fund Project of Southwest University of Science and Technology (20zx7130), and Open Fund of Key Laboratory in Luzhou (NJ202201).

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  1. Guiqiang He and Lei Gao have contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China

    Guiqiang He, Lei Gao, Wenjing Jiao, Jiahui Wang, Yanxia Wei & Jian Zhou

  2. Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China

    Guiqiang He, Yanxia Wei & Jian Zhou

  3. Luzhou Vocational and Technical College, Luzhou, 646000, Sichuan, China

    Yue Deng

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  1. Guiqiang He
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He, G., Gao, L., Deng, Y. et al. Microbial succession in different years of pit mud from a distillery in Sichuan for Nong-xiang Baijiu fermentation. Food Sci Biotechnol (2024). https://doi.org/10.1007/s10068-024-01558-4

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