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Application of experimental design as a statistical approach to recover bioactive peptides from different food sources

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Abstract

Bioactive peptides (BAPs) derived from samples of animals and plants have been widely recommended and consumed for their beneficial properties to human health and to control several diseases. This work presents the applications of experimental designs (DoE) used to perform factor screening and/or optimization focused on finding the ideal hydrolysis condition to obtain BAPs with specific biological activities. The collection and discussion of articles revealed that Box Behnken Desing and Central Composite Design were the most used. The main parameters evaluated were pH, time, temperature and enzyme/substrate ratio. Among vegetable protein sources, soy was the most used in the generation of BAPs, and among animal proteins, milk and shrimp stood out as the most explored sources. The degree of hydrolysis and antioxidant activity were the most investigated responses in obtaining BAPs. This review brings new information that helps researchers apply these DoE to obtain high-quality BAPs with the desired biological activities.

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Abbreviations

BAPs:

Bioactive peptides

DoE:

Design of experiments

RSM:

Response surface methodology

OFAT:

One-factor-at-a-time

FFD:

Full factorial design

FrFD:

Fractional factorial design

PBD:

Plackett–Burman design

CCD:

Central composite design

BBD:

Box–Behnken design

SCD:

Simplex centroid design

SLbD:

Simplex lattice design

DH:

Degree of hydrolysis

E/S:

Enzyme/substrate ratio

Da:

Dalton

MW:

Molar weight

MWCO:

Molecular weight cut-off

AAs:

Amino acids

AA:

Antioxidant activity

DPPH:

Diphenyl-picrylhydrazyle

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

FRAP:

Ferric reducing antioxidant power

ORAC:

Oxygen radical absorbance capacity

RP:

Reducing power

DPP-IV:

Dipeptidyl peptidase-IV

IBC:

Iron-binding capacity

TCA:

Trichloroacetic acid

TCA-SPI:

Trichloroacetic acid soluble peptide index

MALDI-TOF–MS:

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry

RP–HPLC–MS/MS:

Reverse-phase high-performance liquid chromatography coupled with tandem mass spectrometry

HPLC-SEC:

Size-exclusion high-performance liquid chromatography

RP-HPLC:

Liquid chromatography and reverse-phase separation

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Acknowledgements

The authors thank to Fundação de Amparo à Pesquisa do Estado do Piauí (FAPEPI, Piauí, Brazil), Fundação de Amparo à Pesquisa do Estado do Maranhão (FAPEMA, Maranhão, Brazil, UNIVERSAL-01600/18), Coordenação de Aperfeiçoamento de Pessoal Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant 400893/2019-3, 140212/2020-5, 200275/2020-8), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant 2022/10416-9) for scholarships and financial support.

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Authors and Affiliations

  1. Departamento de Química, Universidade Federal do Piauí – UFPI, P.O. Box 64049-550, Teresina, PI, Brazil

    Mikael Kélvin de Albuquerque Mendes, Christian Bremmer dos Santos Oliveira, Carla Mariana da Silva Medeiros, Cícero Alves Lopes Júnior & Edivan Carvalho Vieira

  2. Departamento de Química, Universidade Estadual do Maranhão – UEMA, P.O. Box, 65604-380, Caxias, MA, Brazil

    Clecio Dantas

  3. Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, 13566-590, Brazil

    Emanuel Carrilho

  4. Embrapa Pecuária Sudeste, São Carlos, São Paulo, 13560-970, Brazil

    Ana Rita de Araujo Nogueira

Authors
  1. Mikael Kélvin de Albuquerque Mendes
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  2. Christian Bremmer dos Santos Oliveira
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  3. Carla Mariana da Silva Medeiros
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  4. Clecio Dantas
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  5. Emanuel Carrilho
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  6. Ana Rita de Araujo Nogueira
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  7. Cícero Alves Lopes Júnior
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  8. Edivan Carvalho Vieira
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Contributions

Mikael Kélvin de Albuquerque Mendes: conceptualization, investigation, writing of the original article. Christian Bremmer dos Santos Oliveira: conceptualization, investigation, writing of the original article. Carla Mariana da Silva Medeiros: conceptualization, investigation, writing of the original article. Clecio Dantas: editing the final version of the article and reviewing the design of the experiments topic. Ana Rita de Araújo Nogueira: rationale, supervision, editing, and correction of the final version of the article. Emanuel Carrilho: rationale, supervision, editing, and revision of the final version of the article. Cícero Alves Lopes Júnior: supervision, acquisition of financing, editing, and correction of the article's final version. Edivan Carvalho Vieira: supervision, acquisition of financing, editing, and correction of the article’s final version.

Corresponding authors

Correspondence to Cícero Alves Lopes Júnior or Edivan Carvalho Vieira.

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de Albuquerque Mendes, M.K., dos Santos Oliveira, C.B., da Silva Medeiros, C.M. et al. Application of experimental design as a statistical approach to recover bioactive peptides from different food sources. Food Sci Biotechnol 33, 1559–1583 (2024). https://doi.org/10.1007/s10068-024-01540-0

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  • DOI: https://doi.org/10.1007/s10068-024-01540-0

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