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Sambou Bamboo salt™ down-regulates the expression levels of angiotensin-converting enzyme 2 in activated human mast cells

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Abstract

Mast cells have a detrimental impact on coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Sambou Bamboo salt™ (BS) suppresses mast cell-mediated inflammatory response and enhances immunity. In this study, we investigated the regulatory effects of BS on expression of angiotensin-converting enzyme 2 (ACE2) and transmembrane protease/serine subfamily member 2 (TMPRSS2) in human mast cell line (HMC)-1 cells. BS resulted in significant reductions in expression levels of ACE2 and TMPRSS2 in activated HMC-1 cells. Levels of tryptase were reduced by BS. In addition, BS blocked activation of activator protein 1 (AP-1), c-Jun NH2-terminal kinases (JNK), p38, and phosphatidylinositide-3-kinase (PI3K) in activated HMC-1 cells. Therefore, these results show that BS reduces levels of ACE2, TMPRSS2, and tryptase by inhibiting AP-1/JNK/p38/PI3K signaling pathways in mast cells. These findings can serve as valuable foundational data for the development of therapeutic agents aimed at preventing SARS-CoV-2 infection.

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Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2022R1F1A1063893).

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Author notes

  1. Ho‑Geun Kang and Yu‑Jin Choi have contributed equally to this work.

Authors and Affiliations

  1. Department of Bio-Convergence System, Graduate School, Hoseo University, 20 Hoseo-Ro, 79 Beon-Gil, Baebang-Eup, Asan, 31499, Republic of Korea

    Ho‑Geun Kang & Hyun‑Ja Jeong

  2. Department of Food Science & Technology, Hoseo University, 20 Hoseo-Ro, 79 Beon-Gil, Baebang-Eup, Asan, 31499, Republic of Korea

    Yu‑Jin Choi & Hyun‑Ja Jeong

  3. BioChip Research Center, Hoseo University, 20 Hoseo-Ro, 79 Beon-Gil, Baebang-Eup, Asan, 31499, Republic of Korea

    Hee‑Yun Kim & Hyun‑Ja Jeong

  4. Department of Science in Korean Medicine, Kyung Hee University, Seoul, 02447, Korea

    Hyung‑Min Kim

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Mast cells have a detrimental impact on coronavirus disease 2019 (COVID-19) induced by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

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Kang, H., Choi, Y., Kim, H. et al. Sambou Bamboo salt™ down-regulates the expression levels of angiotensin-converting enzyme 2 in activated human mast cells. Food Sci Biotechnol 33, 1697–1705 (2024). https://doi.org/10.1007/s10068-023-01438-3

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