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An overview of various methods for in vitro biofilm formation: a review

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Abstract

Biofilms are widely present in the natural environment and are difficult to remove as they are a survival strategy of microorganisms. Thus, the importance of studying biofilms is being increasingly recognized in food, medical, dental, and water quality-related industries. While research on biofilm detection methods is actively progressing, research on biofilm formation is not progressing rapidly. Moreover, there are few standardized methods because biofilm formation is affected by various factors. However, comprehensive knowledge of biofilm formation is essential to select a suitable method for research purposes. To better understand the various in vitro biofilm formation methods, the principles and characteristics of each method are explained in this review by dividing the methods into static and dynamic systems. In addition, the applications of biofilm research based on various assays are also discussed.

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Acknowledgements

This research was supported by the Ministry of Food and Drug Safety (22192MFDS024) in 2022.

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  1. Department of Food and Nutrition, Chung-Ang University, 4726 Seodong-dearo, Anseong-si, Gyeonggi-do, 17546, Republic of Korea

    Areum Han & Sun-Young Lee

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Han, A., Lee, SY. An overview of various methods for in vitro biofilm formation: a review. Food Sci Biotechnol 32, 1617–1629 (2023). https://doi.org/10.1007/s10068-023-01425-8

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