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Fermentative optimization and characterization of exopolysaccharides from Enterococcus faecium F58 isolated from traditional fresh goat cheese

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Abstract

This study focused on optimizing the fermentation-based production of Exopolysaccharides (EPS) from Enterococcus faecium F58 initially isolated from traditional Moroccan Jben, a fresh goat cheese. Using the central composite design, yeast extract, MnSO4, and time affect EPS concentration. The highest experimental and predicted EPS production yields were 2.46 g/L ± 0.38 and 2.86 g/L, respectively. Optimal concentrations of yeast extract (4.46 g/L) and MnSO4 (0.011 g/L) were identified after 26 h at 30 °C. Characterization of EPS was conducted using SEM with EDX, XRD, and FTIR analyses. These tests revealed a specific morphology and an amorphous structure. Additionally, thermogravimetric analysis indicated adequate EPS stability up to 200 °C with anti-adhesion properties against different pathogens. This study offers valuable insights into the optimized production of EPS from Enterococcus faecium F58, which exhibits significant structural and functional properties for various applications in the food and biotechnology industries.

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Acknowledgements

The author M. Z. was the beneficiary of a scholarship from the National Center for Scientific and Technical Research [Grant Number 18UIZ2016].

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Authors and Affiliations

  1. LASIME Research Laboratory, Bioprocess and Environment Team, Agadir Superior School of Technology, Ibn Zohr University, BP 33/S, 80150, Agadir, Morocco

    Mariem Zanzan, Youssef Ezzaky & Fouad Achemchem

  2. Laboratory of Microbial Biotechnology and Vegetal Protection, Faculty of Sciences, University Ibn Zohr, BP 32/S, 80000, Agadir, Morocco

    Mariem Zanzan & Fatima Hamadi

  3. Department of Food Science and Technology, UIC Zoonosis y Enfermedades Emergentes (ENZOEM), CeiA3, Universidad de Córdoba, Campus Rabanales, 14014, Córdoba, Spain

    Antonio Valero

  4. Biotechnology, Materials & Environment Team, Faculty of Sciences, University Ibn Zohr, BP 32/S, 80000, Agadir, Morocco

    Rachid Mamouni

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  1. Mariem Zanzan
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Correspondence to Fouad Achemchem.

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Zanzan, M., Ezzaky, Y., Achemchem, F. et al. Fermentative optimization and characterization of exopolysaccharides from Enterococcus faecium F58 isolated from traditional fresh goat cheese. Food Sci Biotechnol 33, 1195–1205 (2024). https://doi.org/10.1007/s10068-023-01424-9

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