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Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms

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Abstract

Biofilm is one of the major problems in food industries and is difficult to be removed or prevented by conventional sanitizers. In this review, we discussed the extracellular matrix-degrading enzymes as a strategy to control biofilms of foodborne pathogenic and food-contaminating bacteria. The biofilms can be degraded by using the enzymes targeting proteins, polysaccharides, extracellular DNA, or lipids which mainly constitute the extracellular polymeric substances of biofilms. However, the efficacy of enzymes varies by the growth medium, bacterial species, strains, or counterpart microorganisms due to a high variation in the composition of extracellular polymeric substances. Several studies demonstrated that the combined treatment using conventional sanitizers or multiple enzymes can synergistically enhance the biofilm removal efficacies. In this review, the application of the immobilized enzymes on solid substrates is also discussed as a potential strategy to prevent biofilm formation on food contact surfaces.

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Acknowledgements

This research was supported by the Main Research Program (E0210702-02) of the Korea Food Research Institute (KFRI), funded by the Ministry of Science and ICT.

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Authors and Affiliations

  1. Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Joo-Sung Kim, Min-Cheol Lim, Se-Min Kim & Joo-Young Lee

  2. Department of Food Biotechnology, Korea University of Science and Technology, 217, Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea

    Joo-Sung Kim & Min-Cheol Lim

  3. Department of Food Science and Technology, Jeonbuk National University, 567, Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do, 54896, Republic of Korea

    Se-Min Kim

  4. Department of Food Science and Biotechnology, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do, 16419, Republic of Korea

    Joo-Young Lee

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  1. Joo-Sung Kim
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Kim, JS., Lim, MC., Kim, SM. et al. Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms. Food Sci Biotechnol 32, 1745–1761 (2023). https://doi.org/10.1007/s10068-023-01373-3

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  • DOI: https://doi.org/10.1007/s10068-023-01373-3

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