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Amperometric bienzymatic biosensor in flow injection analysis system for determination of aspartame in foods

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Abstract

An amperometric bienzymatic biosensor was developed for the determination of aspartame in a flow injection analysis (FIA) system, consisting of two enzyme reactor columns packed with immobilized α-chymotrypsin (CHY) and alcohol oxidase (AOX) beads and a hydrogen peroxide electrode, connected in series. The CHY and AOX were separately immobilized on glutaraldehyde (GA)-activated beads through covalent bonding. The biosensor fabrication and operational conditions were optimized. The optimal fabrication conditions were: 2% GA with 120 min activation time; and 250 U/mL CHY and 100 U/mL AOX, with 180 min enzyme immobilization time. The optimal operational conditions were a flow rate of 0.5 mL/min and pH 8.0 at room temperature. The developed biosensor showed linearity over the aspartame concentration range 0.01–1.2 mM, with a detection limit of 0.005 mM. The developed biosensor was satisfactorily applied for detecting aspartame in beverage samples without any excessive pretreatments.

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Acknowledgements

This research was funded by Kasetsart University, Bangkok, Thailand through the Graduate School Fellowship Program.

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  1. Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand

    Tanaporn Tangtawewipat & Saipin Thanachasai

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  1. Tanaporn Tangtawewipat
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Tangtawewipat, T., Thanachasai, S. Amperometric bienzymatic biosensor in flow injection analysis system for determination of aspartame in foods. Food Sci Biotechnol 33, 343–354 (2024). https://doi.org/10.1007/s10068-023-01347-5

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