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Bacillus subtilis SPB1 lipopeptide biosurfactant: antibacterial efficiency against the phytopathogenic bacteria Agrobacterium tumefaciens and compared production in submerged and solid state fermentation systems

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Abstract

Bacillus subtilis SPB1 derived biosurfactants (BioS) proved its bio-control activity against Agrobacterium tumefaciens using tomato plant. Almost 83% of disease symptoms triggered by Agrobacterium tumefaciens were reduced. Aiming potential application, we studied lipopeptide cost-effective production in both fermentations systems, namely the submerged fermentation (SmF) and the solid-state fermentation (SSF) as well as the use of Aleppo pine waste and confectionery effluent as cheap substrates. Optimization studies using Box–Behnken (BB) design followed by the analysis with response surface methodology were applied. When using an effluent/sea water ratio of 1, Aleppo pine waste of 14.08 g/L and an inoculum size of 0.2, a best production yield of 17.16 ± 0.91 mg/g was obtained for the SmF. While for the SSF, the best production yield of 27.59 ± 1.63 mg/g was achieved when the value of Aleppo pine waste, moisture, and inoculum size were, respectively, equal to 25 g, 75%, and 0.2. Hence, this work demonstrated the superiority of SSF over SmF.

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All data and materials are available.

Abbreviations

BB:

Box Behnken

BioS:

Biosurfactants

SmF:

Submerged fermentation

SSF:

Solid state fermentation

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Acknowledgements

The Ministry of Higher Education, Scientific Research, and Technology of Tunisia provided grants in support of this work. The authors want to sincerely thank Moulin TRIKI Group for their support.

Funding

The work is funded by the Ministry of Higher Education and Scientific Research and the Higher Education-Tunisia-Protection of Plants Researcher.

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Authors and Affiliations

  1. Laboratoire d’Amélioration des Plantes et Valorisation des Agro-Ressources, Ecole Nationale d’Ingénieurs de Sfax, Sfax, Tunisie

    Mouna Bouassida & Dhouha Ghribi

  2. Bioréacteur couple à un ultra filtre, Ecole Nationale D’Ingénieurs de Sfax, Sfax, Tunisie

    Mouna Bouassida

  3. Laboratoire de Biochimie et Génie Enzymatique des Lipases, Ecole Nationale d’Ingénieurs de Sfax, Sfax, Tunisie

    Inès Mnif

  4. Faculté des Sciences de Gabes, Université de Gabes, Gabes, Tunisie

    Inès Mnif

  5. Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam, 31441, Saudi Arabia

    Ines Hammami

  6. Institut de l’Olivier-Institution of Agricultural Research and Higher Education-Tunisia-Protection of Plants Researcher, Tunis, Tunisie

    Mohamed-Ali Triki

  7. Institut Supérieur de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisie

    Dhouha Ghribi

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  1. Mouna Bouassida
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  2. Inès Mnif
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  3. Ines Hammami
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  4. Mohamed-Ali Triki
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The first author of the present manuscript Dr MB realized and writes this paper. The second author Dr IM helped in the redaction of the paper. Dr IH helped in the realization of the experiences. Professor MAT and Professor DG helped in the elaboration of the plan of this work and corrected this paper.

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Correspondence to Inès Mnif.

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Bouassida, M., Mnif, I., Hammami, I. et al. Bacillus subtilis SPB1 lipopeptide biosurfactant: antibacterial efficiency against the phytopathogenic bacteria Agrobacterium tumefaciens and compared production in submerged and solid state fermentation systems. Food Sci Biotechnol 32, 1595–1609 (2023). https://doi.org/10.1007/s10068-023-01274-5

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