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Properties and anti-inflammatory effects of Sargassum muticum enzymatic extracts decomposed using crude enzyme from Shewanella oneidensis PKA1008

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Abstract

This study was conducted to investigate the general properties of an enzymatic extract of Sargassum muticum (SM) produced using a crude enzyme from Shewanella oneidensis PKA 1008 and their anti-inflammatory activities. The SM was mixed with crude enzymes from S. oneidensis PKA 1008 (1:1 (v/v)) and incubated at 30 °C for 0, 3, 6, 12, 24, 48, and 60 h. S. oneidensis PKA 1008 crude enzyme showed the highest SM enzymatic extracts degradation ability when reacted with SM for 48 h. These evaluations demonstrated a 134.25% increase in reducing sugar content and a 14.90% reduction in viscosity at 48 h. The pH, lightness (L*) and yellowness (b*) of the SM enzymatic extracts decreased significantly with increasing reaction time. Moreover, the SM enzymatic extracts demonstrated significant anti-inflammatory activity. These results indicate that the crude enzyme from S. oneidensis PKA 1008 can be used to enhance the polysaccharide degradation of SM, and the resultant oligosaccharides may have an anti-inflammatory effect.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2012R1A6A1028677).

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Authors and Affiliations

  1. Department of Food Science & Technology, Institute of Food Science, Pukyong National University, 48513, Busan, Republic of Korea

    Ji-Eun Lee, Xiaotong Xu, Woo-Sin Kang, Si-Hyeong Ryu, Han-Ho Kim, Su-Ryong Kim, Min-Ji Kim & Dong-Hyun Ahn

  2. Institute of Fisheries Sciences, Pukyong National University, 46041, Busan, Republic of Korea

    So-Mi Jeong & Ga-Hye Lee

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  1. Ji-Eun Lee
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Lee, JE., Xu, X., Jeong, SM. et al. Properties and anti-inflammatory effects of Sargassum muticum enzymatic extracts decomposed using crude enzyme from Shewanella oneidensis PKA1008. Food Sci Biotechnol 31, 1299–1307 (2022). https://doi.org/10.1007/s10068-022-01103-1

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