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Metabolites of oregano (Origanum vulgare) seed and their anti-obesity effects on 3T3-L1 adipocytes through down-regulated adipogenesis

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Abstract

Metabolites of the 80% ethanol extract (OSE) and ethyl acetate fraction (OSEA) of oregano seed were analyzed by GC–MS, and anti-obesity effects of OSE and OSEA were evaluated in 3T3-L1 adipocyte. OSE possessed high content of glucose, fructofuranose, and sucrose while OSEA had high content of phenolic chemicals. OSEA contained higher levels of gallic acid, syringic acid, protocatechuic acid, and catechin than OSE. OSEA inhibited lipid droplet accumulation with concentration dependent manner in 3T3-L1 preadipocytes during differentiation. OSEA showed more inhibition ability than OSE by 13.7-fold at the level of 125 μg/mL. Additionally, relative mRNA and protein expression levels of pparγ, c/ebpα, fas, and srebp-1c which are related to adipogenesis were significantly lower in OSEA treatment group than in OSE treatment group (p < 0.05). Therefore, OSEA could be used as anti-obesity functional ingredient.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF- 2021R1F1A1060533).

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  1. Department of Food and Nutrition, College of Health Science, Kangwon National University, Samcheok, Gangwon, 25949, Korea

    Hyun-Jong Lee & Mi-Ja Kim

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  1. Hyun-Jong Lee
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Correspondence to Mi-Ja Kim.

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Lee, HJ., Kim, MJ. Metabolites of oregano (Origanum vulgare) seed and their anti-obesity effects on 3T3-L1 adipocytes through down-regulated adipogenesis. Food Sci Biotechnol 31, 867–878 (2022). https://doi.org/10.1007/s10068-022-01085-0

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