Skip to main content
Log in

Increased shelf life of Oncorhynchus mykiss (Rainbow trout) through Cu-Clay nanocomposites

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Microbial growth is widely responsible for shortened shelf life of cold water-living fish products. So, it seems that current chemical-based food packaging has no acceptable efficacy, and food industrialists tend to the usage of more novel approaches like active food packaging. Among them, there is a great research interest in nanotechnology-emerging approaches. This study aimed to investigate the anti-microbial efficacies of Polyethylene/CuNP/nanoclay nanocomposites to enhance the shelf life and physiochemical features of rainbow trout. Three main nanocomposites with various concentrations of Cu and clay nanoparticles were examined. SEM, XRD, and EDX (as physiochemical analysis), disk diffusion (as antimicrobial assays), total volatile nitrogen (TVB-N), and peroxide value (PV) (as biochemical parameters) were measured. Based on the results, nanocomposites could reduce the microorganism growth rate by reducing the number of colonies (33.3%), inhibitory activities against both gram-positive (8 mm) and gram-negative bacteria (10 mm), maintenance of TVB-N (42% reduction), and PV (44% reduction) below the standard range. To sum up, these new nanocomposites can be a good candidate to enhance the shelf life of Rainbow Trout.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abdollahi M, Rezaei M, Farzi G. A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan. Journal of Food Engineering 111(2): 343–350 (2012)

    Article  CAS  Google Scholar 

  • Abdou ES, Osheba A, Sorour M. Effect of chitosan and chitosan-nanoparticles as active coating on microbiological characteristics of fish fingers. International Journal of Applied 2(7): 158–169 (2012)

  • Abou-Elela JM, Farag AM. Bacteriological quality and metal contents of Diplodus vuljaris and Siganus rivuloyus in the eastern harbour water: A comparative study of freshly harvested and market fish. Egyptian Journal of Aquatic Research 30: 216–225 (2004)

    Google Scholar 

  • Alfei S, Marengo B, Zuccari G. Nanotechnology application in food packaging: A plethora of opportunities versus pending risks assessment and public concerns. Food Research International 137: 109664 (2020)

    Article  CAS  PubMed  Google Scholar 

  • Arashisar Ş, Hisar O, Kaya M, Yanik T. Effects of modified atmosphere and vacuum packaging on microbiological and chemical properties of rainbow trout (Oncorynchus mykiss) fillets. International Journal of Food Microbiology 97(2): 209–214 (2004)

    Article  CAS  PubMed  Google Scholar 

  • Arfat YA, Ejaz M, Jacob H, Ahmed J. Deciphering the potential of guar gum/Ag-Cu nanocomposite films as an active food packaging material. Carbohydrate Polymers 157: 65–71 (2017)

    Article  CAS  PubMed  Google Scholar 

  • Avella M, De Vlieger JJ, Errico ME, Fischer S, Vacca P, Volpe MG. Biodegradable starch/clay nanocomposite films for food packaging applications. Food Chemistry 93(3): 467–474 (2005)

    Article  CAS  Google Scholar 

  • Balakrishnan H, Hassan A, Wahit MU, Yussuf A, Razak SBA. Novel toughened polylactic acid nanocomposite: mechanical, thermal and morphological properties. Materials & Design 31(7): 3289–3298 (2010)

    Article  CAS  Google Scholar 

  • Barani S, Ahari H, Bazgir S. Increasing the shelf life of pikeperch (Sander lucioperca) fillets affected by low-density polyethylene/Ag/TiO2 nanocomposites experimentally produced by sol-gel and melt-mixing methods. International Journal of Food Properties 21(1): 1923–1936 (2018)

    Article  CAS  Google Scholar 

  • Barriuso B, Astiasarán I, Ansorena D. A review of analytical methods measuring lipid oxidation status in foods: a challenging task. European Food Research and Technology 236(1): 1–15 (2013)

    Article  CAS  Google Scholar 

  • Beigmohammadi F, Peighambardoust SH, Hesari J, Azadmard-Damirchi S, Peighambardoust SJ, Khosrowshahi NK. Antibacterial properties of LDPE nanocomposite films in packaging of UF cheese. LWT-Food Science and Technology 65: 106–111 (2016)

    Article  CAS  Google Scholar 

  • Biemer JJ. Antimicrobial susceptibility testing by the Kirby-Bauer disc diffusion method. Annals of Clinical & Laboratory Science 3(2): 135–140 (1973)

    CAS  Google Scholar 

  • Bondoc I, Şindilar E-V. Controlul sanitar veterinar al calităţii şi salubrităţii alimentelor: Editura" Ion Ionescu de la Brad" (2002)

  • Bondoc I. The veterinary sanitary control of fish and fisheries products. Control of Products and Food of Animal Origin (Controlul Produselorsi Alimentelor De Origine Animală-Original Title) 1: 264–346 (2014)

    Google Scholar 

  • Bumbudsanpharoke N, Ko S. Nanoclays in food and beverage packaging. Journal of Nanomaterials (2019)

  • Cai S, Pourdeyhimi B, Loboa EG. High-throughput fabrication method for producing a silver-nanoparticles-doped nanoclay polymer composite with novel synergistic antibacterial effects at the material interface. ACS Applied Materials & Interfaces 9(25): 21105–21115 (2017)

    Article  CAS  Google Scholar 

  • Campos CA, Gerschenson LN, Flores SK. Development of edible films and coatings with antimicrobial activity. Food and Bioprocess Technology 4(6): 849–875 (2011)

    Article  CAS  Google Scholar 

  • Choi RN, Cheigh CI, Lee SY, Chung MS. Preparation and properties of polypropylene/clay nanocomposites for food packaging. Journal of Food Science 76(8): N62–N67 (2011)

    Article  CAS  PubMed  Google Scholar 

  • D'Amico S, Collins T, Marx J-C, Feller G, Gerday C. Psychrophilic microorganisms: challenges for life. EMBO Reports 7(4): 385–389 (2006)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Davachi SM, Shekarabi AS. Preparation and characterization of antibacterial, eco-friendly edible nanocomposite films containing Salvia macrosiphon and nanoclay. International Journal of Biological Macromolecules 113: 66–72 (2018)

    Article  CAS  PubMed  Google Scholar 

  • De Kievit TR, Parkins MD, Gillis RJ, Srikumar R, Ceri H, Poole K, Iglewski BH, Storey DG. Multidrug efflux pumps: expression patterns and contribution to antibiotic resistance in Pseudomonas aeruginosa biofilms. Antimicrobial Agents and Chemotherapy 45(6): 1761–1770 (2001)

    Article  PubMed  PubMed Central  Google Scholar 

  • Demitri C, De Benedictis VM, Madaghiele M, Corcione CE, Maffezzoli A. Nanostructured active chitosan-based films for food packaging applications: effect of graphene stacks on mechanical properties. Measurement 90: 418–423 (2016)

    Article  Google Scholar 

  • Falguera V, Quintero JP, Jiménez A, Muñoz JA, Ibarz A. Edible films and coatings: structures, active functions and trends in their use. Trends in Food Science & Technology 22(6): 292–303 (2011)

    Article  CAS  Google Scholar 

  • Ghasemian E, Naghoni A, Rahvar H, Kialha M, Tabaraie B. Evaluating the effect of copper nanoparticles in inhibiting Pseudomonas aeruginosa and Listeria monocytogenes biofilm formation. Jundishapur Journal of Microbiology 8(5): e17430 (2015)

  • Gordon MH. 7 - Factors affecting lipid oxidation pp. 128-141. In: Understanding and Measuring the Shelf life of Food. Steele R (ed). Woodhead Publishing (2004)

  • Gounot AM. Psychrophilic and psychrotrophic microorganisms. Experientia 42(11): 1192–1197 (1986)

    Article  CAS  PubMed  Google Scholar 

  • Haaland H, Njaa LR. Ammonia (NH3) and total volatile nitrogen (TVN) in preserved and unpreserved stored, whole fish. Journal of the Science of Food and Agriculture 44(4): 335–342 (1988)

    Article  CAS  Google Scholar 

  • Haaland H, Njaa LR. Total volatile nitrogen—A quality criterion for fish silage?. Aquaculture 79(1): 311–316 (1989)

    Article  CAS  Google Scholar 

  • Ionel B. European regulation in the veterinary sanitary and food safety area, a component of the European policies on the safety of food products and the protection of consumer interests: A 2007 retrospective. Part two: Regulations. Universul Juridic (Suplim): 16–19 (2018)

  • Jongjareonrak A, Benjakul S, Visessanguan W, Tanaka M. Fatty acids and their sucrose esters affect the properties of fish skin gelatin-based film. European Food Research and Technology 222(5): 650–657 (2006)

    Article  CAS  Google Scholar 

  • Kalpana S, Priyadarshini SR, Maria Leena M, Moses JA, Anandharamakrishnan C. Intelligent packaging: Trends and applications in food systems. Trends in Food Science & Technology 93: 145–157 (2019)

    Article  CAS  Google Scholar 

  • Kanatt SR. Development of active/intelligent food packaging film containing Amaranthus leaf extract for shelf life extension of chicken/fish during chilled storage. Food Packaging and Shelf Life 24: 100506 (2020)

    Article  Google Scholar 

  • Karlin K, Itoh S, Rokita S. Copper-Oxygen Chemistry (2011)

  • Khalaj M-J, Ahmadi H, Lesankhosh R, Khalaj G. Study of physical and mechanical properties of polypropylene nanocomposites for food packaging application: Nano-clay modified with iron nanoparticles. Trends in Food Science & Technology 51: 41–48 (2016)

    Article  CAS  Google Scholar 

  • Khoshnoudi-Nia S, Moosavi-Nasab M. Nondestructive determination of the total volatile basic nitrogen (TVB-N) content using hyperspectral imaging in Japanese threadfin bream (Nemipterusjaponicus) fillet. Iranian Journal of Nutrition Sciences & Food Technology 15(1): 113–122 (2020)

    Google Scholar 

  • Li M, Ma Z, Zhu Y, Xia H, Yao M, Chu X, Wang X, Yang K, Yang M, Zhang Y, Mao C. Toward a molecular understanding of the antibacterial mechanism of copper-bearing Titanium alloys against Staphylococcus aureus. Advanced Healthcare Materials 5(5): 557–566 (2016)

    Article  CAS  PubMed  Google Scholar 

  • Llorens A, Lloret E, Picouet P, Fernandez A. Study of the antifungal potential of novel cellulose/copper composites as absorbent materials for fruit juices. International Journal of Food Microbiology 158(2): 113–119 (2012a)

    Article  CAS  PubMed  Google Scholar 

  • Llorens A, Lloret E, Picouet PA, Trbojevich R, Fernandez A. Metallic-based micro and nanocomposites in food contact materials and active food packaging. Trends in Food Science & Technology 24(1): 19–29 (2012b)

    Article  CAS  Google Scholar 

  • Lomate GB, Dandi B, Mishra S. Development of antimicrobial LDPE/Cu nanocomposite food packaging film for extended shelf life of peda. Food Packaging and Shelf Life 16: 211–219 (2018)

    Article  Google Scholar 

  • Maghami M, Motalebi AA, Anvar SAA. Influence of chitosan nanoparticles and fennel essential oils (Foeniculum vulgare) on the shelf life of Huso huso fish fillets during the storage. Food Science & Nutrition 7(9): 3030–3041 (2019)

    Article  CAS  Google Scholar 

  • Mari A, Antonini G. Validation of the micro biological survey method for total viable count and E. coli in food samples. American Journal of Food Technology 6(11): 951–962 (2011)

    Article  Google Scholar 

  • Mohammadzadeh-Vazifeh M, Hosseini SM, Mohammadi A, Jahanfar M, Maleki H. Investigation of the antimicrobial properties of nanoclay and chitosan based nanocomposite on the microbial characteristics of Gouda cheese. Iranian journal of microbiology 12(2): 121–126 (2020)

    PubMed  PubMed Central  Google Scholar 

  • Molinaro S, Romero MC, Boaro M, Sensidoni A, Lagazio C, Morris M, Kerry J. Effect of nanoclay-type and PLA optical purity on the characteristics of PLA-based nanocomposite films. Journal of Food Engineering 117(1): 113–123 (2013)

    Article  CAS  Google Scholar 

  • Morihama ACD, Mierzwa JC. Clay nanoparticles effects on performance and morphology of poly(vinylidene fluoride) membranes. Brazilian Journal of Chemical Engineering 31: 79–93 (2014)

    Article  Google Scholar 

  • Morsy M, Khalaf H, Sharoba A, El-Tanahi H. Applicability of biosensor and oxygen sensor for monitoring spoilage and bacterial contaminants of packed minced beef and poultry. Paper read at Proceedings of the 2nd International Conference on Biotechnology Applications in Agriculture (ICBAA), Hurghada, Egypt (2014)

  • Normanno G, Firinu A, Virgilio S, Mula G, Dambrosio A, Poggiu A, Decastelli L, Mioni R, Scuota S, Bolzoni G, Di Giannatale E, Salinetti AP, La Salandra G, Bartoli M, Zuccon F, Pirino T, Sias S, Parisi A, Quaglia NC, Celano GV. Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy. International Journal of Food Microbiology 98(1): 73–79 (2005)

    Article  CAS  PubMed  Google Scholar 

  • Ozdemir M, Floros JD. Active food packaging technologies. Critical reviews in food science and nutrition 44(3): 185–193 (2004)

    Article  CAS  PubMed  Google Scholar 

  • Özyurt G, Özkütük AS, Şimşek A, Yeşilsu AF, Ergüven M. Quality and shelf life of cold and frozen rainbow trout (Oncorhynchus mykiss) fillets: effects of fish protein-based biodegradable coatings. International Journal of Food Properties 18(9): 1876–1887 (2015)

    Article  Google Scholar 

  • Pandey S, Jana KK, Aswal VK, Rana D, Maiti P. Effect of nanoparticle on the mechanical and gas barrier properties of thermoplastic polyurethane. Applied Clay Science 146: 468–474 (2017)

    Article  CAS  Google Scholar 

  • Pereira de Abreu DA, Paseiro Losada P, Angulo I, Cruz JM. Development of new polyolefin films with nanoclays for application in food packaging. European Polymer Journal 43(6): 2229–2243 (2007)

    Article  CAS  Google Scholar 

  • Refae R, Nasr N. Microbiological hazard analysis during manufacturing of food packaging materials (2009)

  • Reza Gheisari H, Aminlari M, Shahram Shekarforoush S. A comparative study of the biochemical and functional properties of camel and cattle meat during frozen storage. Veterinarski Arhiv 79(1): 51–68 (2009)

    Google Scholar 

  • Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomaterialia 4(3): 707–716 (2008)

    Article  CAS  PubMed  Google Scholar 

  • Saravanakumar K, Sathiyaseelan A, Mariadoss AVA, Xiaowen H, Wang M-H. Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application. International Journal of Biological Macromolecules 153: 207–214 (2020b)

    Article  CAS  PubMed  Google Scholar 

  • Saravanakumar K, Sathiyaseelan A, Mariadoss AVA, Xiaowen H, Wang M-H. Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application. International Journal of Biological Macromolecules (2020a)

  • Shams B, Ebrahimi NG, Khodaiyan F. Development of antibacterial nanocomposite: whey protein-gelatin-nanoclay films with orange peel extract and tripolyphosphate as potential food packaging. Advances in Polymer Technology (2019)

  • Sitthisak S, Knutsson L, Webb JW, Jayaswal RK. Molecular characterization of the copper transport system in Staphylococcus aureus. Microbiology 153(12): 4274–4283 (2007)

    Article  CAS  PubMed  Google Scholar 

  • Souza VG, Pires JR, Vieira ÉT, Coelhoso IM, Duarte MP, Fernando AL. Shelf life assessment of fresh poultry meat packaged in novel bionanocomposite of chitosan/montmorillonite incorporated with ginger essential oil. Coatings 8(5): 177 (2018)

    Article  Google Scholar 

  • Stark C, McNeil D, Savage G. The effect of storage conditions on the stability of peroxide values of New Zealand grown walnuts. Proceedings of the Nutrition Society of New Zealand (2000)

  • Suttiponparnit K, Jiang J, Sahu M, Suvachittanont S, Charinpanitkul T, Biswas P. Role of surface area, primary particle size, and crystal phase on titanium dioxide nanoparticle dispersion properties. Nanoscale Research Letters 6(1): 27 (2011)

    Article  PubMed  Google Scholar 

  • Taheri A, Anvar SAA, Ahari H, Fogliano V. Comparison the functional properties of protein Hydrolysates from poultry byproducts and rainbow trout. IFRO 12(1): 154–169 (2013)

    Google Scholar 

  • Tas CE, Unal H. Thermally buffering polyethylene/halloysite/phase change material nanocomposite packaging films for cold storage of foods. Journal of Food Engineering 292: 110351 (2021)

    Article  CAS  Google Scholar 

  • Tian F, Decker EA, McClements DJ, Goddard JM. Influence of non-migratory metal-chelating active packaging film on food quality: Impact on physical and chemical stability of emulsions. Food Chemistry 151: 257–265 (2014)

    Article  CAS  PubMed  Google Scholar 

  • Tornuk F, Hancer M, Sagdic O, Yetim H. LLDPE based food packaging incorporated with nanoclays grafted with bioactive compounds to extend shelf life of some meat products. LWT-Food Science and Technology 64(2): 540–546 (2015)

    Article  CAS  Google Scholar 

  • Warheit DB, Reed KL, Sayes CM. A role for nanoparticle surface reactivity in facilitating pulmonary toxicity and development of a base set of hazard assays as a component of nanoparticle risk management. Inhalation Toxicology 21(sup1): 61–67 (2009)

    Article  CAS  PubMed  Google Scholar 

  • Weaver L, Noyce JO, Michels HT, Keevil C.W. Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus. Journal of Applied Microbiology 109(6): 2200–2205 (2010)

    Article  CAS  PubMed  Google Scholar 

  • Xia Y, Rubino M, Auras R. Chapter Seven - Interaction of nanoclay-reinforced packaging nanocomposites with food simulants and compost environments pp. 275-298. In: Advances in Food and Nutrition Research, Lim L-T, Rogers M (ed). Academic Press, Cambridge (2019)

  • Xue B, Jiang Y, Liu D. Preparation and characterization of a novel anticorrosion material: Cu/LLDPE nanocomposites. Materials Transactions 52(1): 96–101 (2011)

    Article  CAS  Google Scholar 

  • Yang D, Yang G, Liang G, Guo Q, Li Y, Li J. High-surface-area disperse silica nanoparticles prepared via sol-gel method using L-lysine catalyst and methanol/water co-solvent. Colloids and Surfaces A: Physicochemical and Engineering Aspects 125700 (2020)

  • Zhong Y, Janes D, Zheng Y, Hetzer M, De Kee D. Mechanical and oxygen barrier properties of organoclay‐polyethylene nanocomposite films. Polymer Engineering & Science 47(7): 1101–1107 (2007)

    Article  CAS  Google Scholar 

  • Zhong T, Oporto GS, Jaczynski J. 19 - Antimicrobial food packaging with cellulose-copper nanoparticles embedded in thermoplastic resins. pp. 671–702. In: Food Preservation, Grumezescu AM (ed). Academic Press, Cambridge (2017)

Download references

Acknowledgements

All of the authors and co-authors who meet the criteria for authorship (according to the journal’s instruction) are mentioned on the title page. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors, and all was done by personal finances.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hamed Ahari.

Ethics declarations

Conflict of interest

All of the authors and co-authors declare that there is no conflict of interest regarding the concept and publication of this article. There are not any non-financial and other competing interest disclosures.

Human and animal rights

This article does not contain any studies with human or animal subjects performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 13 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Azari, A., Ahari, H. & Anvar, A.A. Increased shelf life of Oncorhynchus mykiss (Rainbow trout) through Cu-Clay nanocomposites. Food Sci Biotechnol 31, 295–309 (2022). https://doi.org/10.1007/s10068-022-01031-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-022-01031-0

Keywords

Navigation