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Properties and bioavailability assessment of shrimp astaxanthin loaded liposomes

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Abstract

This study aimed to investigate the effects of phospholipid composition on the properties and bioavailability of astaxanthin-loaded liposomes using cell culture. Two mixtures of phospholipids with different proportions of phosphatidylcholine (PC, 23% and 70%) were used at various concentrations (0.8, 1.6, and 2.0% w/v) to prepare astaxanthin-loaded liposomes, which were investigated for entrapment efficiency (EE), antioxidant activity, morphology and changes in astaxanthin properties during a storage period of 8 weeks at 4 °C. Furthermore, Caco-2 human colon adenocarcinoma cells were employed to examine the cellular uptake of astaxanthin-loaded liposomes. The highest EE was observed with astaxanthin-loaded liposomes containing 70% PC, and used at the concentration of 2.0% w/v. Liposomes maintained the antioxidant activity of astaxanthin. All liposomal preparations were non-toxic. Cellular uptake of astaxanthin-loaded liposomes containing 70% PC was significantly higher than that of 23% PC-containing liposomes (p < 0.05).

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Acknowledgements

The authors thank the Center for Advanced Studies for Agriculture and Food, Kasetsart University under the Higher Education Research Promotion and National Research University Project of Thailand; Kasetsart University Research and Development Institute and also Cerebos Awards for financial support.

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Authors and Affiliations

  1. Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand

    Anantita Sangsuriyawong & Wanwimol Klaypradit

  2. Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand

    Anantita Sangsuriyawong & Wanwimol Klaypradit

  3. Department of Food Technology, Faculty of Science, Siam University, Bangkok, Thailand

    Maruj Limpawattana

  4. Institute of Food Research and Product Development, Kasetsart University, Bangkok, Thailand

    Dalad Siriwan

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  1. Anantita Sangsuriyawong
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  2. Maruj Limpawattana
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  3. Dalad Siriwan
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  4. Wanwimol Klaypradit
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Correspondence to Wanwimol Klaypradit.

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Sangsuriyawong, A., Limpawattana, M., Siriwan, D. et al. Properties and bioavailability assessment of shrimp astaxanthin loaded liposomes. Food Sci Biotechnol 28, 529–537 (2019). https://doi.org/10.1007/s10068-018-0495-x

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  • DOI: https://doi.org/10.1007/s10068-018-0495-x

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