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Glycerides isolated from the aerial parts of Malva verticillata cause immunomodulation effects via splenocyte function and NK anti-tumor activity

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Abstract

A preliminary study revealed that a 10 µg/mL n-BuOH fraction of Malva verticillata aerial parts significantly enhanced splenocyte proliferation and induced significant enhancement of natural-killer (NK) cell activity against tumor cells (YAC-1). This study was initiated to identify the principal components that exhibited these activities, and four glycerides were isolated through repeated SiO2 and ODS column chromatography. Structures of compounds 1–4 were determined to be (2S)-1-O-palmitoyl glyceride, (2S)-1-O-stearoyl glyceride, (2S)-1-O-linolenoyl glyceride, and (2S)-1,2-di-O-linoleoyl glyceride, respectively. Compounds 1–3 showed potential immune-enhancing activity in murine splenocyte and natural-killer (NK) cells at 10 µM. In contrast, compound 4 showed weak activity, indicating the monoacyl glycerides (1–3) are more effective than diacyl glyceride (4). Also, the longer the carbon number of the fatty acid in monoacyl glyceride, the better the activity, and the monoacyl glyceride including an unsaturated fatty acid (3) is more effective than the glycerides including the saturated fatty acids (1–2).

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Acknowledgements

This work was supported by the Korea Evaluation Institute of Industrial Technology (Development of novel materials based on dual functions for improvement of atopic dermatitis and photoaging by regulating NFAT Grant number: 10076337), Republic of Korea.

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Authors and Affiliations

  1. Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung-Hee University, Yongin, 17104, Republic of Korea

    Jung-Hwan Ko, Rodrigo Castaneda, Sun-Woo Joo, Hyoung-Geun Kim, Yeong-Geun Lee, Tong Ho Kang & Nam-In Baek

  2. Department of Horticultural Biotechnology, Graduate School of Biotechnology, Kyung-Hee University, Yongin, 17104, Republic of Korea

    Youn-Hyung Lee

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  1. Jung-Hwan Ko
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  2. Rodrigo Castaneda
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  3. Sun-Woo Joo
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  6. Youn-Hyung Lee
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Correspondence to Nam-In Baek.

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Ko, JH., Castaneda, R., Joo, SW. et al. Glycerides isolated from the aerial parts of Malva verticillata cause immunomodulation effects via splenocyte function and NK anti-tumor activity. Food Sci Biotechnol 27, 1023–1030 (2018). https://doi.org/10.1007/s10068-018-0344-y

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  • DOI: https://doi.org/10.1007/s10068-018-0344-y

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