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Anti-oxidant and anti-adipogenic effects of acorn (Quercus acutissima CARR.) shell extracts via regulation of wnt signaling in 3T3-L1 cells

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Abstract

Acorn (Quercus acutissima CARR.) is a nut from the Fagaceae family that has been used in traditional medicine for many years. However, shells from acorns are regarded as a by-product and are mostly discarded. Anti-adipogenic activities of acorn shells were investigated using 3T3-L1 cells and methanol shell extracts (AE-M). AE-M demonstrated Cu2+-chelation activities and anti-oxidant activities via reduction of oxidative stress levels induced using AAPH. Six days after adipocyte differentiation, 50 and 100 μg/mL AE-M completely suppressed 3T3-L1 adipogenesis and the anti-adipogenic effect was stronger than for the positive control 50 μM quercetin. Treatment with AE-M in 3T3-L1 cells reduced mRNA expression levels of adipogenic genes. AE-M-inhibition was found in pre-adipogenic, early, and intermediate stages of adipogenesis in 3T3-L1 cells. The Wnt/β-catenin signaling pathway is required for AE-M-inhibition of 3T3-L1 adipogenesis.

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Authors and Affiliations

  1. Traditional and Biomedical Research Center, Daejeon, 34520, Korea

    Ji-Hye Song

  2. Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Kasugai, 487-8501, Japan

    Masayuki Kamegai & Yasuyuki Ishida

  3. Research Institute for Biological Functions, Chubu University, Kasugai, 487-8501, Japan

    Byung-Yoon Cha

  4. Department of Food, Nutrition and Biotechnology, Kyungnam University, Changwon, Gyeongnam, 51767, Korea

    Myung-Soo Shon, Si-Kyung Kim, Seung-Cheol Lee & Gyo-Nam Kim

Authors
  1. Myung-Soo Shon
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  2. Si-Kyung Kim
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  3. Ji-Hye Song
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  4. Masayuki Kamegai
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Correspondence to Gyo-Nam Kim.

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Shon, MS., Kim, SK., Song, JH. et al. Anti-oxidant and anti-adipogenic effects of acorn (Quercus acutissima CARR.) shell extracts via regulation of wnt signaling in 3T3-L1 cells. Food Sci Biotechnol 25, 875–882 (2016). https://doi.org/10.1007/s10068-016-0144-1

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  • DOI: https://doi.org/10.1007/s10068-016-0144-1

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