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Anti-obesity effect of grape skin extract in 3T3-L1 adipocytes

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Abstract

The aim of the present study was to determine the effects of grape skin which was well known as natural antioxidant using 3T3-L1 adipocyte, representative cells with morphological and biochemical characteristics of adipocytes. Grape skin extract (GSE) suppressed the differentiation of 3T3-L1 adipocytes, and decreased the triglycerides content with lipid accumulation. The adipogenesis inhibitory effect of GSE was confirmed in adipocyte hormone secretion, such as leptin and adiponectin, as well as glycerol-3-phosphate dehydrogenase activity. However, apoptosis of both mature pre-adipocytes and adipocytes was not induced by GSE. The level of gene and protein expression was observed to understand the antiobesity mechanism underlying GSE. GSE treatment of 3T3-L1 cells resulted in decrease in the adipogenic transcription factor (PPARγ, C/EBPα, and SREBP1) gene and protein expression, and preadipocyte secreted factor-1 mRNA was up-regulated compared to the adipocyte control. The lipolysis-related mRNA level (hormone-sensitive lipase, lipoprotein lipase, and perilipin) in adipocytes with the addition of grape skin was similar to preadipocytes. These results support the potential application of GSE as a natural anti-obesity material in the food industry.

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Authors and Affiliations

  1. Department of Food Science and Technology, Catholic University of Daegu, Daegu, 712-702, Korea

    Kwang-Sup Youn & Joo-Heon Hong

  2. Bio Industry Center, Daegu Technopark, Daegu, 704-701, Korea

    Yoo Seok Jeong & Hee Kyoung Jung

  3. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, Korea

    Kyung-Hyun Cho

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  1. Yoo Seok Jeong
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  2. Hee Kyoung Jung
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Correspondence to Joo-Heon Hong.

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Jeong, Y.S., Jung, H.K., Cho, KH. et al. Anti-obesity effect of grape skin extract in 3T3-L1 adipocytes. Food Sci Biotechnol 20, 635–642 (2011). https://doi.org/10.1007/s10068-011-0090-x

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