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Production of xylooligosaccharides from corncobs using ultrasound-assisted enzymatic hydrolysis

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Abstract

Xylooligosaccharides were obtained using ultrasound-assisted enzymatic hydrolysis from xylan that was alkali extracted from corncobs. Effects of alkali treatments on xylan extraction were investigated. The influence of operational conditions in ultrasound-assisted enzymatic hydrolysis on xylooligosaccharide production was also studied. Optimal conditions for xylan extraction were determined to be an alkaline concentration of 15%, a corncobs to alkaline solution ratio of 1:20, a reaction temperature of 100°C, and a reaction time of 2 h. Optimal conditions for xylanase-catalyzed hydrolysis of xylan to xylooligosaccharides were determined to be an enzyme concentration of 4 g/L, an ultrasound power of 200 W, a reaction temperature of 50°C and a reaction time of 10 min. Using a combination of ultrasound and enzyme increased the reducing sugar content by approximately 50%, compared with using of enzymes alone. Ultrasound and enzymes exert a synergistic effect.

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Authors and Affiliations

  1. Henan Postdoctoral Research Base, Food and Bioengineering College, Xuchang University, Xuchang, 461000, China

    Juntao Sun, Zhichao Zhang, Fugang Xiao & Ximei Jin

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  1. Juntao Sun
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  2. Zhichao Zhang
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  3. Fugang Xiao
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  4. Ximei Jin
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Correspondence to Juntao Sun.

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Sun, J., Zhang, Z., Xiao, F. et al. Production of xylooligosaccharides from corncobs using ultrasound-assisted enzymatic hydrolysis. Food Sci Biotechnol 24, 2077–2081 (2015). https://doi.org/10.1007/s10068-015-0276-8

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  • DOI: https://doi.org/10.1007/s10068-015-0276-8

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