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Preparation and characterization of acid and pepsin-soluble collagens from scales of croceine and redlip croakers

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Abstract

Acid-soluble (ASC) and pepsin-soluble collagen (PSC) from scales of croceine croaker (ASC-PC and PSC-PC) and redlip croaker (ASC-PP and PSC-PP) were prepared with yields of 0.37±0.08% (ASCPC), 1.09±0.23% (PSC-PC), 0.42±0.09% (ASC-PP), and 1.14±0.30% (PSC-PP) (dry scale basis). Gly (347.1-359.8 residues/1,000 residues) was the major amino acid. Contents of imino acid were between 189.4 and 192.9 residues/1,000 residues in all 4 collagens. ASC-PC and ASC-PP were type I collagens. Differences in subunit components between ASC and PSC were observed. Denaturation temperature values of ASC-PC, ASC-PP, PSC-PC, and PSC-PP were 30.7, 30.1, 27.5, and 26.6°C, respectively. All 4 collagens were soluble at pH 1-4. Values declined when NaCl concentrations exceeded 2%. Freeze-dried collagens showed loose, fibrous, and porous structures. The 4 scale collagens can be alternatives to collagen from terrestrial animals for applications in functional food, cosmetic, and biomedical industries.

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Authors and Affiliations

  1. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, 316022, China

    Qian-Qian Wu, Tao Li, Bin Wang & Guo-Fang Ding

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  1. Qian-Qian Wu
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  2. Tao Li
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  3. Bin Wang
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  4. Guo-Fang Ding
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Correspondence to Bin Wang.

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Wu, QQ., Li, T., Wang, B. et al. Preparation and characterization of acid and pepsin-soluble collagens from scales of croceine and redlip croakers. Food Sci Biotechnol 24, 2003–2010 (2015). https://doi.org/10.1007/s10068-015-0264-z

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  • DOI: https://doi.org/10.1007/s10068-015-0264-z

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