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Characterization of acid-soluble collagens from the cartilages of scalloped hammerhead (Sphyrna lewini), red stingray (Dasyatis akajei), and skate (Raja porosa)

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Abstract

Acid-soluble collagen from the cartilages of scalloped hammerhead (Sphyrna lewini, ASC-S), red stingray (Dasyatis akajei, ASC-D), and skate (Raja porosa, ASC-R) were isolated with yields of 5.64±0.41, 8.72±0.31, and 6.74±0.28% on the basis of wet weight, respectively. ASC-S, ASC-D, and ASC-R had glycine as the major amino acid with contents of 220, 231, and 228 residues/1,000 residues, respectively, and contained imino acid of 174, 155, and 142 residues/1,000 residues, respectively. Three kinds of collagens more likely were comprised of 2 types of collagen (type I and II) and had some differences in primary structures. The denaturation temperatures (Tds) of the ASC-S, ASC-D, and ASC-R were 23.8, 15.1, and 12.1°C, respectively. Maximum solubility of the 3 ASCs in 0.5 M acetic acid was observed at pH 2, and a sharp decrease in solubility above 2%(w/v) was observed in the presence of NaCl. The 3 lyophilized collagens displayed loose, fibrous, and porous structures.

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Authors and Affiliations

  1. National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science, Zhejiang Ocean University, Qixiangtai Road 51, Zhoushan, 316004, China

    Chang-Feng Chi

  2. School of Food and Pharmacy, Zhejiang Ocean University, Qixiangtai Road 51, Zhoushan, 316004, China

    Bin Wang, Zhong-Rui Li, Hong-Yu Luo & Guo-Fang Ding

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  1. Chang-Feng Chi
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  2. Bin Wang
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  3. Zhong-Rui Li
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  5. Guo-Fang Ding
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Correspondence to Bin Wang.

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Chi, CF., Wang, B., Li, ZR. et al. Characterization of acid-soluble collagens from the cartilages of scalloped hammerhead (Sphyrna lewini), red stingray (Dasyatis akajei), and skate (Raja porosa). Food Sci Biotechnol 22, 909–916 (2013). https://doi.org/10.1007/s10068-013-0163-0

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  • DOI: https://doi.org/10.1007/s10068-013-0163-0

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