Skip to main content
SpringerLink
Log in

Protective effect of carvacrol from Thymus quinquecostatus Celak against tert-butyl hydroperoxide-induced oxidative damage in Chang cells

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Carvacrol is a monoterpenic phenol present in Thymus quinquecostatus Celak. The protective effects of carvacrol against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage were investigated in human Chang cells. Cells treated with carvacrol extracts promoted Chang cell survival and protection was associated with stabilization of the mitochondrial membrane potential (MMP), prevention of oxidative stress-triggered reactive oxygen species (ROS), and lipid peroxidation (MDA production). In addition, Annexin V/PI, observed using Hoechst staining, indicated that carvacrol inhibited t-BHP-induced cell damage and stimulated the antioxidant capability of Chang cells due to elevation of glutathione (GSH) levels, which were reduced by t-BHP treatment. Carvacrol prevented oxidative stress-induced Chang cell damage via suppression of ROS and MDA levels, and increased GSH levels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Loguercio C, Federico A. Oxidative stress in viral and alcoholic hepatitis. Free Radical Bio. Med. 34: 1–10 (2003)

    Article  CAS  Google Scholar 

  2. Arteel GE, Kadiiska MB, Rusyn I, Bradford BU, Mason RP, Raleigh JA, Thurman RG. Oxidative stress occurs in perfused rat liver at low oxygen tension by mechanisms involving peroxynitrite. Mol. Pharmacol. 55: 708–715 (1999)

    CAS  Google Scholar 

  3. Hoek JB, Cahill A, Pastorino JG. Alcohol and mitochondria: A dysfunctional relationship. Gastroenterology 122: 2049–2063 (2002)

    Article  CAS  Google Scholar 

  4. Kevin LG, Camara AK, Riess ML, Novalija E, Stowe DF. Ischemic preconditioning alters real-time measure of O2 radicals in intact hearts with ischemia and reperfusion. Am. J. Physiol.-Heart C. 284: H566–H574 (2003)

    Article  CAS  Google Scholar 

  5. Kakkar P, Singh BK. Mitochondria: A hub of redox activities and cellular distress control. Mol. Cell. Biochem. 305: 235–253 (2007)

    Article  CAS  Google Scholar 

  6. Kim JA, Kang YS, Kim YO, Lee SH, Lee YS. Role of Ca2+ influx in the tert-butyl hydroperoxide-induced apoptosis of HepG2 human hepatoblastoma cells. Exp. Mol. Med. 30: 137–144 (1998)

    Article  CAS  Google Scholar 

  7. Dumont P, Burton M, Chen QM, Gonos ES, Frippiat C, Mazarati JB, Eliaers F, Remacle J, Toussaint O. Induction of replicative senescence biomarkers by sublethal oxidative stresses in normal human fibroblast. Free Radical Bio. Med. 28: 361–373 (2000)

    Article  CAS  Google Scholar 

  8. Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH. Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem. Toxicol. 38: 411–416 (2000)

    Article  CAS  Google Scholar 

  9. Lin WL, Wang CJ, Tsai YY, Liu CL, Hwang JM, Tseng TH. Inhibitory effect of esculetin on oxidative damage induced by t-butyl hydroperoxide in rat liver. Arch. Toxicol. 74: 467–472 (2000)

    Article  CAS  Google Scholar 

  10. Minotti G, Borrello S, Palombini G, Galeotti T. Cytochrome P-450 deficiency and resistance to t-butyl hydroperoxide of hepatoma microsomal lipid peroxidation. Biochim. Biophys. Acta 876: 220–225 (1986)

    Article  CAS  Google Scholar 

  11. Zeytinoglu H, Incesu Z, Baser KH. Inhibition of DNA synthesis by carvacrol in mouse myoblast cells bearing a human N-RAS oncogene. Phytomedicine 10: 292–299 (2003)

    Article  CAS  Google Scholar 

  12. Kirimer N, Baser KHC, Tümen G. Carvacrol rich plants in Turkey. Chem. Nat. Compd. 31: 37–41 (1995)

    Article  Google Scholar 

  13. Liang WZ, Chou CT, Lu T, Chi CC, Tseng LL, Pan CC, Lin KL, Kuo CC, Jan CR. The mechanism of carvacrol-evoked [Ca2+]i rises and non-Ca2+-triggered cell death in OC2 human oral cancer cells. Toxicology 303: 152–161 (2013)

    Article  CAS  Google Scholar 

  14. Ultee A, Smid EJ. Influence of carvacrol on growth and toxin production by Bacillus cereus. Int. J. Food Microbiol. 64: 373–378 (2001)

    Article  CAS  Google Scholar 

  15. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18: 414–420 (2007)

    Article  CAS  Google Scholar 

  16. Kim YS, Lee SJ, Hwang JW, Kim EK, Kim SE, Kim EH, Moon SH, Jeon BT, Park PJ. In vitro protective effects of Thymus quinquecostatus Celak extracts on t-BHP-induced cell damage through antioxidant activity. Food Chem. Toxicol. 50: 4191–4198 (2012)

    Article  CAS  Google Scholar 

  17. Hotta M, Nakata R, Katsukawa M, Hori K, Takahashi S, Inoue H. Carvacrol, a component of thyme oil, activates PPARα and γ, and suppresses COX-2 expression. J. Lipid Res. 51: 132–139 (2010)

    Article  Google Scholar 

  18. Arunasree KM. Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine 17: 581–588 (2010)

    Article  CAS  Google Scholar 

  19. Guimarães AG, Oliveira GF, Melo MS, Cavalcanti SC, Antoniolli AR, Bonjardim LR, Silva FA, Santos JP, Rocha RF, Moreira JC, Araújo AA, Gelain DP, Quintans-Júnior LJ. Bioassay-guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic Clin. Pharmacol. 107: 949–957 (2010)

    Article  Google Scholar 

  20. Melo FH, Moura BA, de Sousa DP, de Vasconcelos SM, Macedo DS, Fonteles MM, Viana GS, de Sousa FC. Antidepressant-like effect of carvacrol (5-isopropyl-2-methylphenol) in mice: Involvement of dopaminergic system. Fund. Clin. Pharmacol. 25: 362–367 (2011)

    Article  CAS  Google Scholar 

  21. Melo FH, Venâncio ET, de Sousa DP, de Franca Fonteles MM, de Vasconcelos SM, Viana GS, de Sousa FC. Anxiolytic-like effect of Carvacrol (5-isopropyl-2-methylphenol) in mice: Involvement with GABAergic transmission. Fund. Clin. Pharmacol. 24: 437–443 (2010)

    Article  CAS  Google Scholar 

  22. Aydin Y, Kutlay O, Ari S, Duman S, Uzuner K, Aydin S. Hypotensive effects of carvacrol on the blood pressure of normotensive rats. Planta Med. 73: 1365–1371 (2007)

    Article  CAS  Google Scholar 

  23. Aristatile B, Al-Numair KS, Veeramani C, Pugalendi KV. Antihyperlipidemic effect of carvacrol on d-galactosamine-induced hepatotoxic rats. J. Basic Clin. Physiol. Pharmacol. 20: 15–27 (2009)

    Article  CAS  Google Scholar 

  24. Kim EK, Je JY, Lee SJ, Kim YS, Hwang JW, Sung SH, Moon SH, Jeon BT, Kim SK, Jeon YJ, Park PJ. Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells. Bioorg. Med. Chem. Lett. 22: 6136–6138 (2012)

    Article  CAS  Google Scholar 

  25. Zamzami N, Maisse C, Metivier D, Kroemer G. Measurement of membrane permeability and permeability transition of mitochondria. Method. Cell Biol. 80: 327–340 (2007)

    Article  CAS  Google Scholar 

  26. Kamal AA, Gomaa A, el Khafif M, Hammad AS. Plasma lipid peroxides among workers exposed to silica or asbestos dusts. Environ. Res. 49: 173–180 (1989)

    Article  CAS  Google Scholar 

  27. Zhang JH, Xu M. DNA fragmentation in apoptosis. Cell Res. 10: 205–211 (2000)

    Article  CAS  Google Scholar 

  28. Kung G, Konstantinidis K, Kitsis RN. Programmed necrosis, not apoptosis, in the heart. Circ. Res. 108: 1017–1036 (2011)

    Article  CAS  Google Scholar 

  29. Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Bio. Med. 9: 515–540 (1990)

    Article  CAS  Google Scholar 

  30. Wu SJ, Ng LT. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia linn, var abbreviata Ser.) in Taiwan. LWT-Food Sci. Technol. 41: 323–330 (2007)

    Article  Google Scholar 

  31. Rhee SG, Chae HZ, Kim K. Peroxiredoxins: A historical overview and speculative preview of novel mechanisms and emerging concepts in cell signaling. Free Radical. Bio. Med. 38: 1543–1552 (2005)

    Article  CAS  Google Scholar 

  32. Higuchi Y. Glutathione depletion-induced chromosomal DNA fragmentation associated with apoptosis and necrosis. J. Cell Mol. Med. 8: 455–464 (2004)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Yon-Suk Kim, Jin-Woo Hwang & Pyo-Jam Park

  2. Department of Chemistry, Pukyoung National University, Busan, 608-737, Korea

    Sun-Joo Park

  3. Department of Food Science and Biotechnology, Kunsan National University, Gunsan, Jeonbuk, 573-701, Korea

    Yong-Tae Kim

  4. Nokyong Research Center, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Si-Heung Sung, Eun-Kyung Kim, Sang-Ho Moon, Byong-Tae Jeon & Pyo-Jam Park

Authors
  1. Yon-Suk Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jin-Woo Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Si-Heung Sung
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sun-Joo Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yong-Tae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Eun-Kyung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sang-Ho Moon
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Byong-Tae Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Pyo-Jam Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pyo-Jam Park.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, YS., Hwang, JW., Sung, SH. et al. Protective effect of carvacrol from Thymus quinquecostatus Celak against tert-butyl hydroperoxide-induced oxidative damage in Chang cells. Food Sci Biotechnol 24, 735–741 (2015). https://doi.org/10.1007/s10068-015-0095-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0095-y

Keywords

Search

Navigation