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Anti-inflammatory, counteractive, and preventive effects of orally administrated oat extracts on dextran sulfate sodium-induced colitis in mice

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Abstract

The anti-inflammatory effects of oat extracts in vitro and in vivo were determined based on antioxidant functions. To examine the effect of oat extracts in murine macrophage RAW 264.7 cells, MTT assays were used and nitric oxide (NO) production was analyzed based on absorbance and inducible nitric oxide synthase (iNOS) expression levels using Western blot analysis. Immunoglobulin expression levels were determined using an enzyme-linked immunosorbent assay (ELISA). Oat extracts showed significant (p<0.05) antioxidant properties and restored the cell viability of LPS-stimulated RAW 264.7 cells, compared with controls. NO production and iNOS protein levels were also reduced by oat extracts. DSS (3%)-administered mice manifested symptoms similar to human colitis patients, and oat extracts attenuated the symptoms of colitis. Preventive and therapeutic administration of oat extracts used as antioxidants can ameliorate inflammatory conditions in cells and DSS-induced colitis in mice.

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References

  1. Ren JL, Pan JS, Lu YP, Sun PQ, Han JH. Inflammatory signaling and cellular senescence. Cell. Signal. 21: 378–383 (2009)

    Article  CAS  Google Scholar 

  2. Son K, Kim YM. In vivo cisplatin-exposed macrophages increase immunostimulant-induced nitric oxide synthesis for tumor cell killing. Cancer Res. 55: 5524–5527 (1995)

    CAS  Google Scholar 

  3. Tsan MF, Gao B. Endogenous ligands of toll-like receptors. J. Leukocyte Biol. 76: 514–519 (2004)

    Article  CAS  Google Scholar 

  4. Sun GY, Xu JF, Jensen MD, Yu S, Wood WG, Gonzalez FA, Simonyi A, Sun AY, Weisman GA. Phospholipase A(2) inastrocytes-responses to oxidative stress, inflammation, and G protein-coupled receptor agonists. Mol. Neurobiol. 31: 27–41 (2005)

    Article  CAS  Google Scholar 

  5. Velayos FS, Sandborn WJ. Positioning biologic therapy for Crohn’s disease and ulcerative colitis. Curr. Gastroenterol. Rep. 9: 521–527 (2007)

    Article  Google Scholar 

  6. Vilela EG, Torres HO, Martins FP, Ferrari Mde L, Andrade MM, Cunha AS. Evaluation of inflammatory activity in Crohn’s disease and ulcerative colitis. World J. Gastroentero. 18: 872–881 (2012)

    Article  Google Scholar 

  7. Travis S. Advances in therapeutic approaches to ulcerative colitis and Crohn’s disease. Curr. Gastroenterol. Rep. 7: 475–484 (2005)

    Article  Google Scholar 

  8. Sands BE. Therapy of inflammatory bowel disease. Gastroenterology 118: S68–S82 (2000)

    Article  CAS  Google Scholar 

  9. Calixto JB, Campos MM, Otuki MF, Santos AR. Anti-inflammatory compounds of plant origin. Part II. Modulation of pro-inflammatory cytokines, chemokines, and adhesion molecules. Planta Med. 70: 93–103 (2004)

    Article  CAS  Google Scholar 

  10. Fiocchi C. Inflammatory bowel disease: Etiology and pathogenesis. Gastroenterology 115: 182–205 (1998)

    Article  CAS  Google Scholar 

  11. Trachootham D, Lu W, Ogasawara MA, Nilsa RD, Huang P. Redox regulation of cell survival. Antioxid. Redox Sign. 10: 1343–1374 (2008)

    Article  CAS  Google Scholar 

  12. Roessner A, Kuester D, Malfertheiner P, Schneider-Stock R. Oxidative stress in ulcerative colitis-associated carcinogenesis. Pathol. Res. Pract. 204: 511–524 (2008)

    Article  CAS  Google Scholar 

  13. Othman RA, Moghadasian MH, Jones PJ. Cholestreol-lowering effects of oat ß-glucan. Nutr. Rev. 69: 299–309 (2011)

    Article  Google Scholar 

  14. Liu L, Zubik L, Collins FW, Marko M, Meydani M. The antiatherogenic potential of oat phenolic compounds. Atherosclerosis 175: 39–49 (2002)

    Article  Google Scholar 

  15. Singh R, De S, Belkheir A. Avena sativa (oat), a potential neutraceutical and therapeutic agent: An overview. Crit. Rev. Food Sci. 53: 126–144 (2013)

    Article  CAS  Google Scholar 

  16. Silano M, Penas Pozo E, Uberti F, Manferdelli S, Del Pinto T, Felli C, Budelli A, Vincetini O, Restani P. Diversity of oat varieties in eliciting the early inflammatory events in celiac disease. Eur. J. Nutr. 53: 1177–1186 (2013)

    Article  Google Scholar 

  17. Hasnat MA, Pervin M, Lim BO. Acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice. Molecules 18: 6663–6678 (2013)

    Article  Google Scholar 

  18. Materska M, Perucka I. Antioxidant activity of the main phenolic compounds isolated form hot pepper fruit (Capsicum annuum L.). J. Agr. Food Chem. 53: 1750–1756 (2005)

    Article  CAS  Google Scholar 

  19. Jeong CH, Choi GN, Kim JH, Kwak JH, Heo HJ, Shim KH, Cho BR, Bae Y-II, Choi JS. In vitro antioxidative activities and phenolic composition of hot water extract from different parts of Cudrania tricuspidata. Food Ind. Nutr. 14: 283–289 (2009)

    CAS  Google Scholar 

  20. Guo Q, Zhao B, Shen S, Hou J, Hu J, Xin W. ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers. Biochim. Biophys. Acta 1427: 13–23 (1999)

    Article  CAS  Google Scholar 

  21. Fridovich I. Superoxide radical and superoxide dismutases. Annu. Rev. Biochem. 64: 97–112 (1995)

    Article  CAS  Google Scholar 

  22. Mylonas C, Kouretas D. Lipid peroxidation and tissue damage. In Vivo 13: 295–309 (1999)

    CAS  Google Scholar 

  23. Chang LW, Yen WJ, Huang SC, Duh PD. Antioxidant activity of sesame coat. Food Chem. 78: 347–54 (2002)

    Article  CAS  Google Scholar 

  24. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation. Curr. Drug Targets Inflamm. Allergy 4: 471–479 (2005)

    Article  CAS  Google Scholar 

  25. Wirtz S, Neurath MF. Mouse models of inflammatory bowel disease. Adv. Drug Deliver. Rev. 59: 1073–1083 (2007)

    Article  CAS  Google Scholar 

  26. Wahl C, Liptay S, Adler G, Schmid RM. Sulfasalazine: A potent and specific inhibitor of nuclear factor? B. J. Clin. Invest. 101: 1163–1174 (1998)

    Article  CAS  Google Scholar 

  27. Rogler G, Andus T. Cytokines in inflammatory bowel disease. World J. Surg. 22: 382–389 (1998)

    Article  CAS  Google Scholar 

  28. Kanhere A, Hertweck A, Bhatia U, Gokmen MR, Perucha E, Jackson I, Lord GM, Jenner RG. T-bet and GATA3 orchestrate Th1 and Th2 differentiation through lineage-specific targeting of distal regulatory elements. Nat. Commun. 3: 1268 (2012)

    Article  Google Scholar 

  29. Yamada K, Hung P, Park TK, Park PJ, Lim BO. A comparison of the immunostimulatory effects of the medicinal herbs Echinacea, Ashwagandha, and Brahmi. J. Ethnopharmacol. 137: 231–235 (2011)

    Article  Google Scholar 

  30. Kaser A, Zeissig S, Blumberg RS. Inflammatory bowel disease. Annu. Rev. Immunol. 28: 573–621 (2010)

    Article  CAS  Google Scholar 

  31. Jackson LN, Zhou YN, Qui SM, Wang QD, Evers BM. Alternative medicine products as a novel treatment strategy for inflammatory bowel disease. Am. J. Chinese Med. 36: 953–965 (2008)

    Article  Google Scholar 

  32. Matos I, Bento AF, Marcon R, Claudino RF, Clixto JB. Preventive and therapeutic oral administration of the pentacyclic triterpene a, β-amyrin ameliorates dextran sulfate sodium-induced colitis in mice: The relevance of cannabinoid system. Mol. Immunol. 54: 482–492 (2013)

    Article  CAS  Google Scholar 

  33. Farombi EO, Adedara IA, Ajayi BO, Ayepola OR, Egbeme EE. Kolaviron, a natural antioxidant and anti-inflammatory phytochemical prevents dextran sulphate sodium-induced colitis in rats. Basic Clin. Pharmacol. 113: 49–55 (2013)

    Article  CAS  Google Scholar 

  34. Gray DA, Clarke MJ, Baux C, Bunting JP, Salter AM. Antioxidant activity of oat extracts added to human LDL particles and in free radical trapping assays. J. Cereal Sci. 36: 209–218 (2002)

    Article  CAS  Google Scholar 

  35. Khansari N, Shakiba Y, Mahmoudi M. Chronic inflammation and oxidative stress as a major causeof age-related diseases and cancer. Recent Pat. Inflamm. Allergy Drug Discov. 3: 73–80 (2009)

    CAS  Google Scholar 

  36. Perše M, Cerar A. Dextran sodium sulphate colitis mouse model: Traps and tricks. J. Biomed. Biotechnol. 2012: 71867 (2012)

    Google Scholar 

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Authors and Affiliations

  1. Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk University, Chungju, Chungbuk, 380-701, Korea

    Md. Abul Hasnat, Mehnaz Pervin, Sa Ra Park, Yong Chang Jang, Ji Hong Lim, Young Jun Kim & Beong Ou Lim

  2. Department of Herbal Crop Research, NIHHS, RDA, Eumseong, Chungbuk, 369-873, Korea

    Young Ock Kim

  3. Department of Family Medicine, Konkuk University Chungju Hospital, Chungju, Chungbuk, 80-701, Korea

    Hyung Jung Kweon

Authors
  1. Md. Abul Hasnat
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  2. Mehnaz Pervin
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  5. Ji Hong Lim
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  9. Beong Ou Lim
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Correspondence to Beong Ou Lim.

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Hasnat, M.A., Pervin, M., Park, S.R. et al. Anti-inflammatory, counteractive, and preventive effects of orally administrated oat extracts on dextran sulfate sodium-induced colitis in mice. Food Sci Biotechnol 24, 619–628 (2015). https://doi.org/10.1007/s10068-015-0081-4

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  • DOI: https://doi.org/10.1007/s10068-015-0081-4

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