Abstract
Mustard oilcake was used as a novel substrate for phytase production with Rhizopus oligosporus MTCC 556. A mixed substrate (wheat bran:mustard oil cake) was used to optimize different medium components under solid state fermentation at a 62% moisture content, pH 5.5, 30°C, an inoculum level of 15%, and a ratio of the volume of a salt solution to the mass of mixed substrate 1.5 mL:10 g. Different carbon and nitrogen sources were used for production of phytase. Use of the nutritional supplements maltose and ammonium sulfate resulted in high phytase yields. The hydrolytic ability of partially purified phytase was evaluated using 5 different food grains. Wheat flour showed a 43.78% phytic acid reduction, rice flour showed 92% release of inorganic phosphorus, and corn flour showed 81 and 68% releases of soluble proteins and reducing sugars. Thus, phytase is suitable for feed applications.
Similar content being viewed by others
References
Wodzinski RJ, Ullah AHJ. Phytase. Adv. Appl. Microbiol. 42: 263–303 (1996)
Vohra A, Satyanarayana T. Phytases: Microbial sources, production, purification, and purification and potential biotechnological applications. Crit. Rev. Biotechnol. 23: 29–60 (2003)
Shivange AV, Serwe A, Denning A, Roccatano D, Haefner S, Schwaneberg U. Directed evolution of a highly active Yersinia mollaretii phytase. Appl. Microbiol. Biot. 95: 405–418 (2012)
Sutardi G, Buckle KA. Characterization of extra- and intracellular phytases from Rhizopus oligosporus used in tempeh production. Int. J. Food Microbiol. 6: 67–79 (1988)
Casey A, Walsh G. Identification and charecterization of a phytase of potential commercial interest. J. Biotechnol. 110: 313–322 (2004)
Vats P, Banerjee UC. Production studies and catalytic properties of phytase (myo-inositolhexakisphosphate phosphohydrolases): An overview. Enzyme Microb. Tech. 35: 3–14 (2004)
Ramachandran S, Singh SK, Larroche C, Soccol CR, Pandey A. Oilcakes and their biotechnological applications-A review. Bioresource Technol. 98: 2000–2009 (2007)
Heinonen JK, Lahti RJ. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphate. Anal. Biochem. 113: 313–317 (1981)
Garcia-Estepa RM, Guerra-Hernandez E, Garcia-Villanova B. Phytic acid content in milled cereal products and breads. Food Res. Int. 32: 217–221 (1999)
Miller GL. Use of dinitrosalicylic reagent for determination of reducing sugars. Anal. Chem. 31: 426–428 (1959)
Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375–400 (1925)
Lowry OH, Rosenbough NJ, Farr AL, Randall RJ. Protein measurment with the folinphenol reagent. J. Biol. Chem. 193: 265–275 (1951)
Pandey A, Szakacs G, Soccol CR, Rodriguez-Leon JA, Soccol VT. Production purification and properties of microbial phytases. Bioresource Technol. 77: 203–214 (2001)
Pagan JD. Advances in Equine Nutrition. Nottingham University Press, Nottingham, UK. pp. 60–65 (1998)
Moongngarm A, Daomukda N, Khumpika S. Chemical compositions, phytochemicals, and antioxidant capacity of rice bran, rice bran layer, and rice germ. APCBEE Proced. 2: 73–79 (2012)
Ramadoss G, Muthukumar K. Ultrasound assisted ammonia pretreatment of sugarcane bagasse for fermentable sugar production. Biochem. Eng. J. 83: 33–41 (2014)
Roopesh K, Ramachandran S, Nampoothri KM, Szakacs G, Pandey A. Comparasion of phytase production on wheat bran and oilcakes in solid state fermentation by Mucor racemosus. Bioresource Technol. 97: 506–511 (2006)
Rani R, Ghosh S. Production of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization. Bioresoure Technol. 102: 10641–10649 (2011)
Gautam P, Sabu A, Pandey A, Szakacs G, Soccol CR. Microbial production of extra-cellular phytase using polystyrene as inert support. Bioresource Technol. 83: 229–233 (2002)
Pandey A. Solid-state fermentation. Biochem. Eng. J. 13: 81–84 (2003)
Haritha K, Sambasivarao KRS. Phytase production by Rhizopus oligosporus MTCC 556 under submerged fermentation conditions. Asian J. Bio Sci. 4: 270–273 (2010)
Gunashree BS, Venkateswaran G. Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations. J. Ind. Microbiol. Biot. 35: 1587–1596 (2008)
Sabu A, Sarita S, Pandey A, Bogar B, Szakacs G, Soccol CR. Solidstate fermentation for production of phytase by Rhizopus oligosporus. Appl. Biochem. Biotech. 102–103: 251–260 (2002)
Ramachandran S, Roopesh K, Nampoothri KM, Szakacs G, Pandey A. Mixed substrate fermentation for the production of phytase by Rhizopus spp. using oil cakes as substrates. Process Biochem. 40: 1749–1754 (2005)
Bogar B, Szakacs G, Linden JC, Pandey A, Tengerdy RP. Optimization of phytase production by solid substrate fermentation. J. Ind. Microbiol. Biot. 30: 183–189 (2003)
Singh B, Satyanarayana T. Phytase production by a thermophilic mould Sporotrichum thermophile in solid state fermentation and its potential applications. Bioresource Technol. 99: 2824–2830 (2008)
Bhavsar K, Kumar VR, Khire JM. High level phytase production by Aspergillus niger NCIM 563 in solid state culture: Response surface optimization, up-scaling, and its partial characterization. J. Ind. Microbiol. Biot. 38: 1407–1417 (2011)
Surya KK, Vanitha S, Suresh S, Radha KV. Production and optimization of phytase from Rhizopus oligosporus using agro residues by solid state fermentation. Middle East. J. Sci. Res. 17: 1839–1845 (2013)
Ali TH, Mohammed LA, Ali NH. Phytic Acid (Myo-inositol hexakisphosphate) Phosphohydrolase from Streptomyces hygroscopicus NRRL B-1476. J. Biol. Sci. 7: 603–613 (2007)
Mullaney EJ, Daly CB, Ullah AH. Advances in phytase research. Adv. Appl. Microbiol. 47: 157–199 (2000)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Suresh, S., Radha, K.V. Effect of a mixed substrate on phytase production by Rhizopus oligosporus MTCC 556 using solid state fermentation and determination of dephytinization activities in food grains. Food Sci Biotechnol 24, 551–559 (2015). https://doi.org/10.1007/s10068-015-0072-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10068-015-0072-5