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Optimization of phytase production by solid substrate fermentation

  • Original Paper
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract

The production of phytase by three feed-grade filamentous fungi (Aspergillus ficuum NRRL 3135, Mucor racemosus NRRL 1994 and Rhizopus oligosporus NRRL 5905) on four commonly used natural feed ingredients (canola meal, cracked corn, soybean meal, wheat bran) was studied in solid substrate fermentation (SSF). A. ficuum NRRL 3135 had the highest yield [15 IU phytase activity/g dry matter (DM)] on wheat bran. By optimizing the supplementation of wheat bran with starch and (NH4)2SO4, phytase production increased to 25 IU/g DM. Optimization was carried out by Plackett-Burman and central composite experimental designs. Using optimized medium, phytase, phosphatase, alpha-amylase and xylanase production by A. ficuum NRRL 3135 was studied in Erlenmeyer flask and tray SSF. By scaling up SSF from flasks to stationary trays, activities of 20 IU phytase activity/g DM were reproducibly obtained.

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Fig. 1a–d.
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Acknowledgements

The research was supported by USDA/SCRP grant No. 58-3148-1-043 for 2001/2003, partly by Hungarian Science Foundation grant OTKA T 029387 and the Hungarian Ministry of Education grant FKFP 0516/1999.

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Authors and Affiliations

  1. Department of Agricultural Chemical Technology, Technical University of Budapest, Gellert ter 4, 1111 , Budapest, Hungary

    B. Bogar & G. Szakacs

  2. Department of Microbiology, Colorado State University, Ft. Collins, CO 80523-1677, USA

    J. C. Linden & R. P. Tengerdy

  3. Biotechnology Division, Regional Research Laboratory, CSIR, 695 019, Trivandrum, India

    A. Pandey

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  1. B. Bogar
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  2. G. Szakacs
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  3. J. C. Linden
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  4. A. Pandey
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  5. R. P. Tengerdy
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Correspondence to J. C. Linden.

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Bogar, B., Szakacs, G., Linden, J.C. et al. Optimization of phytase production by solid substrate fermentation. J IND MICROBIOL BIOTECHNOL 30, 183–189 (2003). https://doi.org/10.1007/s10295-003-0027-3

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  • DOI: https://doi.org/10.1007/s10295-003-0027-3

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