Skip to main content
SpringerLink
Log in

Anti-atherosclerosis effect of pine nut oil in high-cholesterol and high-fat diet fed rats and its mechanism studies in human umbilical vein endothelial cells

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The objective of this study was to determine the anti-atherosclerotic effects of pine nut oil in rats fed a high-cholesterol and high-fat diet (HCHF). Using a randomized block design, Sprague-Dawley rats were divided into 5 groups and fed experimental diets for 6 weeks (normal diet, HCHF, HCHF+7 or 15% pine nut oil, HCHF+simvastatin). This study shows that markers of atherosclerosis, such as increased liver thiobarbituric acid reactive substance levels, aortic fatty streak area, and low-density lipoprotein cholesterol concentrations, were lower in the HCHF plus pine nut oil groups than in the HCHF group. Conversely, positive cardiovascular indicators, such as increased paraoxonase activity and high-density lipoprotein cholesterol concentrations, were higher in HCHF plus pine nut oil groups than in the HCHF group. This study suggests that pine nut oil improves atherosclerotic markers and could be used as a functional ingredient in the food industry to promote cardiovascular health.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Anderson TJ. Arterial stiffness or endothelial dysfunction as a surrogate marker of vascular risk. Can. J. Cardiol. 22 (Suppl. B): 72B–80B (2006)

    Article  Google Scholar 

  2. Langheinrich AC, Bohle RM. Atherosclerosis: Humoral and cellular factors of inflammation. Virchows Arch. 446: 101–111 (2005)

    Article  CAS  Google Scholar 

  3. Badimon L, Vilahur G. LDL-cholesterol versus HDL-cholesterol in the atherosclerotic plaque: Inflammatory resolution versus thrombotic chaos. Ann. NY Acad. Sci. 1254: 18–32 (2012)

    Article  CAS  Google Scholar 

  4. Fan A, Wu X, Wu H, Li L, Huang R, Zhu Y, Qiu Y, Fu J, Ren J, Zhu C. Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. PLoS ONE 9: e85337 (2014)

    Article  Google Scholar 

  5. Singh V, Sharma R, Kumar A, Deedwania P. Low high-density lipoprotein cholesterol: Current status and future strategies for management. Vasc. Health Risk Manag. 6: 979–996 (2010)

    Article  CAS  Google Scholar 

  6. Del Boccio G, Lapenna D, Porreca E, Penelli A, Savini F, Feliciani P, Ricci G, Cuccunillo F. Aortic antioxidant defence mechanisms: time-related changes in cholesterol-fed rabbits. Atherosclerosis 81: 127–135 (1990)

    Article  Google Scholar 

  7. Kim JK, Kim SH, Noh KH, Jang JH, Song YS. Effects of green tea seed oil on the cholesterol, TBARS, and inflammatory responses in C57BL/6 mice fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 36: 284–290 (2007)

    Article  CAS  Google Scholar 

  8. Hong KH. Dietary therapy for prevention of atherosclerosis. J. Korean Med. Assoc. 52: 287–298 (2009)

    Article  Google Scholar 

  9. Lee SH, Jang MS. Physicochemical properties of jatjook as influenced by various levels of pinenut. Korean J. Soc. Food Sci. 10: 99–103 (1994)

    Google Scholar 

  10. Park YS, Chung MS. Cholesterol-lowering effect of pine nut in plasma of rats. Korean J. Food Sci. Technol. 37: 702–708 (2005)

    Google Scholar 

  11. Yoon TH, Lee SM. Effect on blood lipids and lipoproteins of a supplement of Korean pinenut oil, rich in 5-Olefinic acids, in normocholesterolemic New Zealand white rabbits. Korean J. Nutr. 27: 323–335 (1994)

    Google Scholar 

  12. Aceampong A, Leveque N, Tchapla A, Heron S. Simple complementary liquid chromatography and mass spectrometry approaches for the characterization of triacylglycerols in Pinus koraiensis seed oil. J. Chromatogr. A 1218: 5087–5100 (2011)

    Article  Google Scholar 

  13. Kamal-Eldin A, Mreau RA. Tree nut oils. pp. 127–149. In: Gourmet and Health Promoting Specialty Oils. Moreau RA, Kamal-Eldin A (eds). AOCS Press, Urbana, IL, USA (2009)

    Google Scholar 

  14. Park SY, Lim YS, Shin SH, Han SN. Impact of Korean pine nut oil on weight gain and immune responses in high-fat diet-induced obsess mice. Nutr. Res. Pract. 7: 352–358 (2013)

    Article  CAS  Google Scholar 

  15. Lee KW, Yu KW, Kim KM, Suh HJ, Lee SW, Rhee C. Effect of pine (Pinus koraiensis)-seed oil supplementation on serum lipid composition in rats and immune response in mice. Food Sci. Biotechnol. 13: 358–361 (2004)

    CAS  Google Scholar 

  16. Wolff RL, Bayard CC. Fatty acid composition of some pine seed oils. J. Am. Oil. Chem. Soc. 72: 1043–1046 (1995)

    Article  CAS  Google Scholar 

  17. Harris WS. Linoleic acid and coronary heart disease. Prostag. Leukotr. Ess. 79: 169–171 (2008)

    Article  CAS  Google Scholar 

  18. Perona JS, Rodríguez-Rodríguez R, Ruiz-Gutierrez V. Effects of oleic acid rich oils on aorta lipids and lipoprotein lipase activity of spontaneously hypertensive rats. J. Agr. Food Chem. 53: 7330–7336 (2005)

    Article  CAS  Google Scholar 

  19. Gavini CK, Mukherjee S, Shukla C, Britton SL, Koch LG, Shi H, Novak CM. Leanness and heightened nonresting energy expenditure: Role of skeletal muscle activity thermogenesis. Am. J. Physiol. Endoc. M. 306: E635–E647 (2014)

    CAS  Google Scholar 

  20. Sullivan EL, Koegler FH, Cameron JL. Individual differences in physical activity are closely associated with changes in body weight in adult female rhesus monkeys (Macaca mulatta). Am. J. Physiol. Reg. I. 291: R633–R642 (2006)

    CAS  Google Scholar 

  21. Jung JH, Kim HS. The inhibitory effect of black soybean on hepatic cholesterol accumulation in high cholesterol and high fat dietinduced non-alcoholic fatty liver disease. Food Chem. Toxicol. 60: 404–412 (2013)

    Article  CAS  Google Scholar 

  22. Lu B, Xia D, Huang W, Wu X, Zhang Y, Yao Y. Hypolipidemic effect of bamboo shoot oil (P. pubescens) in Sprague-Dawley rats. J. Food Sci. 75: H205–H211 (2010)

    Article  CAS  Google Scholar 

  23. Chen JH, Tsai CW, Wang CP, Lin HH. Anti-atherosclerotic potential of gossypetin via inhibiting LDL oxidation and foam cell formation. Toxicol. Appl. Pharm. 272: 313–324 (2013)

    Article  CAS  Google Scholar 

  24. Hamilton RL, Williams MC, Field CJ, Havel RJ. Discoidal bilayer structure of nascent high density lipoproteins from perfused rat liver. J. Clin. Invest. 58: 667–680 (1976)

    Article  CAS  Google Scholar 

  25. Nam TG. Lipid peroxidation and its toxicological implications. Toxicol. Res. 27: 1–6 (2011)

    Article  Google Scholar 

  26. Florentin M, Liberopoulos EN, Wierzbicki AS, Mikhailidis DP. Multiple actions of high-density lipoprotein. Curr. Opin. Cardiol. 23: 370–378 (2008)

    Article  Google Scholar 

  27. Watson AD, Berliner JA, Hama SY, La Du BN, Faull KF, Fogelman AM, Navab M. Protective effect of high density lipoprotein associated paraoxonase. Inhibition of the biological activity of minimally oxidized low density lipoprotein. J. Clin. Invest. 96: 2882–2891 (1995)

    Article  CAS  Google Scholar 

  28. Navab M, Hama-Levy S, Van Lenten BJ, Fonarow GC, Carlinez CJ, Castellani LW. Mildly oxidized LDL induces an increased apolipoprotein J/paraoxonase ratio. J. Clin. Invest. 99: 2005–2019 (1997)

    Article  CAS  Google Scholar 

  29. Le NH, Shin S, Tu TH, Kim CS, Kang JH, Tsuyoshi G, Teruo K, Han SN, Yu R. Diet enriched with korean pine nut oil improves mitochondrial oxidative metabolism in skeletal muscle and brown adipose tissue in diet-induced obesity. J. Agr. Food Chem. 60: 11935–11941 (2012)

    Article  CAS  Google Scholar 

  30. Lee JW, Lee KW, Lee SW, Kim IH, Rhee C. Selective increase in pinolenic acid (all-cis-5,9,12-18:3) in Korean pine nut oil by crystallization and its effect on LDL-receptor activity. Lipids 39: 383–387 (2004)

    Article  CAS  Google Scholar 

  31. Zhu M, Chen D, Li D, Ding H, Zhang T, Xu T, Zhang Y. Luteolin inhibits angiotensin II-induced human umbilical vein endothelial cell proliferation and migration through downregulation of Src and Akt phosphorylation. Circ. J. 77: 772–779 (2013)

    Article  CAS  Google Scholar 

  32. Hunt BJ. The endothelium in atherogenesis. Lupus 9: 189–193 (2000)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Kyoung Kon Kim & Myeon Choe

  2. Well-Being Bioproducts RIC Center, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Yun Hwan Kang, Tae Woo Kim & Myeon Choe

Authors
  1. Yun Hwan Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyoung Kon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tae Woo Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Myeon Choe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Myeon Choe.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kang, Y.H., Kim, K.K., Kim, T.W. et al. Anti-atherosclerosis effect of pine nut oil in high-cholesterol and high-fat diet fed rats and its mechanism studies in human umbilical vein endothelial cells. Food Sci Biotechnol 24, 323–332 (2015). https://doi.org/10.1007/s10068-015-0043-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0043-x

Keywords

Search

Navigation