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Khz (fusion of ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in A549 human lung cancer cells by generating reactive oxygen species and decreasing the mitochondrial membrane potential

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Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. The expression of p53, Bax, Bcl-2, and actin proteins was analyzed by western blotting. Khz inhibited cell division and induced apoptosis in A549 cells. Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. Khz reduced MMP and Bcl-2 protein levels, but increased ROS generation and p53 and pro-apoptotic proteins. The anti-proliferative and pro-apoptotic effects of Khz suggest that this extract shows great promise as a potential chemotherapeutic agent.

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Correspondence to Ren Sheng Wang.

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Kim, T.H., Kim, J.S., Kim, Z.H. et al. Khz (fusion of ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in A549 human lung cancer cells by generating reactive oxygen species and decreasing the mitochondrial membrane potential. Food Sci Biotechnol 23, 859–864 (2014).

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