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Eupatorium japonicum extract regulates inflammation through suppression of the TRIF-dependent signaling pathway of toll-like receptors

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Abstract

Inflammation can be mediated by invading microbial pathogens. Toll-like receptors (TLRs) recognize invading microbial pathogens, inducing innate immune responses. Broadly, the activation of TLRs induces two major downstream signaling pathways, myeloid differential factor 88 (MyD88)- and Toll/interleukin 1 receptor domain-containing adapter inducing interferon-β (TRIF)-dependent pathways, which lead to the activation of nuclear factor-κB (NF-κB) and interferon regulatory factor 3 (IRF3). To evaluate the therapeutic potential of the ethanol extracts of flowers of Eupatorium japonicum Thunb (EJE), its effect on signal transduction via the TLR signaling pathways induced by lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly[I:C]) was examined. EJE suppressed the activation of NF-κB and IRF3 induced by LPS or poly[I:C]. EJE also inhibited LPS- or poly[I:C]-induced IRF3 phosphorylation as well as interferon-inducible genes, such as interferon inducible protein-10. These results suggest that EJE can modulate TLR signaling pathways, realizing effective therapeutic options for chronic inflammatory diseases.

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Authors and Affiliations

  1. Departments of Medical Science, College of Medical Sciences, SoonChunHyang University, Chungnam, 336-745, Korea

    Gyo-Jeong Gu, Sang-Il Ahn & Hyung-Sun Youn

  2. Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University, Chungnam, 336-745, Korea

    Se Jin Lim & Hyung-Sun Youn

  3. Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Ji Hun Paek & Soon Sung Lim

  4. Department of Biological Environment, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Songmun Kim

Authors
  1. Gyo-Jeong Gu
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  2. Sang-Il Ahn
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  3. Se Jin Lim
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  4. Ji Hun Paek
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  5. Songmun Kim
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  6. Soon Sung Lim
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  7. Hyung-Sun Youn
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Correspondence to Hyung-Sun Youn.

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Gu, GJ., Ahn, SI., Lim, S.J. et al. Eupatorium japonicum extract regulates inflammation through suppression of the TRIF-dependent signaling pathway of toll-like receptors. Food Sci Biotechnol 23, 587–592 (2014). https://doi.org/10.1007/s10068-014-0080-x

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  • DOI: https://doi.org/10.1007/s10068-014-0080-x

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