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Optimization of nobiletin extraction assisted by microwave from orange byproduct using response surface methodology

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Abstract

Nobiletin (NOB) is a well-known polymethoxylated flavone that has only been found in citrus. The objective of this study is to obtain the optimal conditions for extracting nobiletin from sweet orange residues by microwave-assisted extraction (MAE) using response surface method. By using the Box-Behnken experimental design and SAS software 3 independent variables including solid/solvent ratio, microwave power, and treatment time were examined for the response variable nobiletin extraction yield. The regression Eq. 7 statistically significant (p<0.001) and R2 was 0.9189. Based on our regression result and considering the feasibility of experiment conduction, we chose the following independent variable values as the optimized conditions for the extraction of NOB: 1:21.91 (g/mL) for the solid/solvent ratio, 14.16 min for the treatment time, and 693.72 W for the microwave power. Under these conditions, the nobiletin yield of 267.8 μg/g of byproduct was achieved.

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Authors and Affiliations

  1. College of Food Science, Southwest University, Chongqing, 400716, China

    Yu Zhang, Hongjun Li & Zhifei He

  2. Citrus Research Institute, Chinese Academy of Agricultural Sciences, Southwest University, Chongqing, 400712, China

    Yu Zhang, Huating Dou, Houjiu Wu, Zhigao Sun, Hua Wang, Xuegen Huang & Yaqin Ma

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  1. Yu Zhang
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  2. Hongjun Li
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  3. Huating Dou
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  4. Zhifei He
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  6. Zhigao Sun
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  7. Hua Wang
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  9. Yaqin Ma
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Correspondence to Hongjun Li.

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Zhang, Y., Li, H., Dou, H. et al. Optimization of nobiletin extraction assisted by microwave from orange byproduct using response surface methodology. Food Sci Biotechnol 22 (Suppl 1), 153–159 (2013). https://doi.org/10.1007/s10068-013-0061-5

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  • DOI: https://doi.org/10.1007/s10068-013-0061-5

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