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Establishment of a stable Amaranthus tricolor callus line for production of food colorant

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Abstract

The present study attempts to establish and screen Amaranthus tricolor L. callus for development of a stable callus line with enhanced production of food colorants. The best betacyanin-producing callus was induced on Murashige and Skoog basal medium supplemented with 1-naphthalene acetic acid (NAA) (0.25 mg/L) and 6-benzylaminopurine (BA) (2 mg/L) using stem segment as explants. The callus line (IIT BT/PBT At 11) was maintained up to 24 subculture cycles. Betalain production in callus was detected by reversed phase HPLC and LCMS. In addition to the reported abundant red-violet pigment amaranthin, 2 new yellow pigments and 18 other bioactive phenylpropanoids were detected in the callus line. A novel betaxanthin, methyl derivative of arginine betaxanthin was identified. All pigments were purified by size exclusion chromatography.

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  1. Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, West Bengal, India

    Mousumi Biswas, Shibendu Sekhar Das & Satyahari Dey

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  1. Mousumi Biswas
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  2. Shibendu Sekhar Das
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  3. Satyahari Dey
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Correspondence to Satyahari Dey.

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Biswas, M., Das, S.S. & Dey, S. Establishment of a stable Amaranthus tricolor callus line for production of food colorant. Food Sci Biotechnol 22 (Suppl 1), 1–8 (2013). https://doi.org/10.1007/s10068-013-0041-9

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  • DOI: https://doi.org/10.1007/s10068-013-0041-9

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