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Anti-obesity effect of komulkosiraegi [Gracilaria vermiculophylla (Ohmi) Papenfuss] extract in 3T3-L1 cells

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Abstract

The ingestion of edible seaweed has long been believed to be beneficial to human health due to its numerous biological actions. In the present study, the ethyl acetate fraction of a komulkosiraegi [Gracilaria vermiculophylla (Ohmi) Papenfuss] ethanol extract (GEFr) was found to potently inhibit adipogenesis of 3T3-L1 preadipocytes, decreasing triglycerol accumulation and the expression of peroxisome proliferator-activated receptor γ (PPARγ), members of the CCAAT/enhancer-binding protein (C/EBP) family, and fatty acid binding protein 2 (aP2). In mature adipocytes, GEFr was found to significantly activate AMP-activated protein kinase (AMPK) by activating liver kinase B1 (LKB1) and stimulating intracellular reactive oxygen species generation. The mRNA levels of genes involved in lipid catabolism were up-regulated. Also, GEFr increased lipolysis in a dose-dependent manner. Taken together, these results suggest that GEFr has potential for use in therapies designed to improve obesity.

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Authors and Affiliations

  1. Department of Biology and Research Institute for Basic Science, Jeju National University, Jeju, 690-756, Korea

    Hyo-Min Kim, Seong-Il Kang, Hye-Sun Shin, Youn-Suk Hong, Seung-Woo Kang, Sun-A Yoon, Jeong-Hwan Kim & Se-Jae Kim

  2. Jeju Sasa Industry Development Agency, Jeju National University, Jeju, 690-756, Korea

    Seong-Il Kang, Hee-Chul Ko & Se-Jae Kim

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  1. Hyo-Min Kim
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  2. Seong-Il Kang
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Correspondence to Se-Jae Kim.

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Kim, HM., Kang, SI., Shin, HS. et al. Anti-obesity effect of komulkosiraegi [Gracilaria vermiculophylla (Ohmi) Papenfuss] extract in 3T3-L1 cells. Food Sci Biotechnol 21, 83–89 (2012). https://doi.org/10.1007/s10068-012-0010-8

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  • DOI: https://doi.org/10.1007/s10068-012-0010-8

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